Document Detail

Kinetics of induction and protective effect of heat-shock proteins after cardioplegic arrest.
MedLine Citation:
PMID:  8633950     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Heat-shock proteins are known to enhance cardiac resistance to ischemia. METHODS: To evaluate the kinetics of heat-shock protein 70 in relation to its effect on postischemic recovery of cardiac mechanical (cardiac output) and endothelial function (as percentage increase of coronary flow in response to 5-hydroxytryptamine), isolated rat hearts were subjected to prolonged hypothermic cardioplegic arrest at different intervals ranging from 12 to 96 hours after heat stress (n = 6 in each interval). RESULTS: Immunoblotting showed the maximal level of heat-shock protein 70, 0.65 +/- 0.10 (arbitrary units +/- standard error of the mean), at 24 hours after heat shock and similar values at 26 and 30 hours (p = not significant). Postischemic recovery of cardiac output and endothelial function (percentage of preischemic value +/- standard error of the mean) observed at 24 hours was 74.0 +/- 2.4 and 58.3 +/- 7.2, respectively. Similar values were observed at 26 and 30 hours (p = not significant). CONCLUSIONS: In a protocol mimicking conditions for cardiac transplantation, postischemic recovery of cardiac output and endothelial function was improved when the interval between heat stress and ischemia ranged from 24 to 30 hours. This correlated with an apparently critical amount of heat-shock protein 70.
M Amrani; J Corbett; S Y Boateng; M J Dunn; M H Yacoub
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  61     ISSN:  0003-4975     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-07-02     Completed Date:  1996-07-02     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1407-11; discussion 1411-2     Citation Subset:  AIM; IM    
National Heart & Lung Institute, Heart Science Centre, Harefield Hospital, Middlesex, United Kingdom.
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MeSH Terms
HSP70 Heat-Shock Proteins / pharmacokinetics,  physiology*
Heart / physiology*
Heart Arrest, Induced*
Hypothermia, Induced
Myocardial Reperfusion Injury / physiopathology
Rats, Sprague-Dawley
Time Factors
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins

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