Document Detail


Kinetics of increased deformability of deoxygenated sickle cells upon oxygenation.
MedLine Citation:
PMID:  14507701     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the kinetics of changes in the deformability of deoxygenated sickle red blood cells when they are exposed to oxygen (O(2)) or carbon monoxide. A flow-channel laser diffraction technique, similar to ektacytometry, was used to assess sickle cell deformability after mixing deoxygenated cells with buffer that was partially or fully saturated with either O(2) or carbon monoxide. We found that the deformability of deoxygenated sickle cells did not regain its optimal value for several seconds after mixing. Among density-fractionated cells, the deformability of the densest fraction was poor and didn't change as a function of O(2) pressure. The deformability of cells from the light and middle fraction increased when exposed to O(2) but only reached maximum deformability when equilibrated with supraphysiological O(2) concentrations. Cells from the middle and lightest fraction took several seconds to regain maximum deformability. These data imply that persistence of sickle cell hemoglobin polymers during circulation in vivo is likely, due to slow and incomplete polymer melting, contributing to the pathophysiology of sickle cell disease.
Authors:
Zhi Huang; Leigh Hearne; Cynthia E Irby; S Bruce King; Samir K Ballas; Daniel B Kim-Shapiro
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  85     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-25     Completed Date:  2004-06-08     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2374-83     Citation Subset:  IM    
Affiliation:
Departments of Physics and Chemistry, Wake Forest University, Winston-Salem, North Carolina 27109 USA.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / blood*,  metabolism,  pathology*
Cell Size
Cells, Cultured
Erythrocyte Deformability*
Erythrocytes, Abnormal / metabolism*,  pathology*
Flow Injection Analysis / methods
Hemoglobin, Sickle / metabolism
Oxygen / blood,  metabolism*
Refractometry / methods
Reproducibility of Results
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
HL58091/HL/NHLBI NIH HHS; HL62198/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobin, Sickle; 7782-44-7/Oxygen
Comments/Corrections

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