Document Detail

Kinetics of fatty acid binding ability of glycated human serum albumin.
MedLine Citation:
PMID:  16184009     Owner:  NLM     Status:  MEDLINE    
Kinetics of fatty acid binding ability of glycated human serum albumin (HSA) were investigated by fluorescent displacement technique with 1-anilino-8-naphtharene sulphonic acid (ANS method), and photometric detection of nonesterified-fatty-acid (NEFA method). Changing of binding affinities of glycated HSA toward oleic acid, linoleic acid, lauric acid, and caproic acid, were not observed by the ANS method. However, decreases of bind-ing capacities after 55 days glycation were confirmed by the NEFA method in comparison to control HSA. The decrease in binding affinities was: oleic acid (84%), linoleic acid (84%), lauric acid (87%), and caproic acid (90%), respectively. The decreases were consistent with decrease of the intact lysine residues in glycated HSA. The present observation indicates that HSA promptly loses its binding ability to fatty acid as soon as the lysine residues at fatty acid binding sites are glycated.
Eiji Yamazaki; Minoru Inagaki; Osamu Kurita; Tetsuji Inoue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biosciences     Volume:  30     ISSN:  0250-5991     ISO Abbreviation:  J. Biosci.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-26     Completed Date:  2008-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  475-81     Citation Subset:  IM    
Industrial Research Division, Science and Technology Promotion Center,Mie Prefecture, Takachaya 5-5-45, Tsu, Mie 514-0819, Japan.
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MeSH Terms
Amino Acids / genetics
Fatty Acids / metabolism*
Glucose / metabolism*
Oleic Acid / metabolism
Sequence Analysis, Protein
Serum Albumin / genetics,  metabolism*
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids; 0/Serum Albumin; 112-80-1/Oleic Acid; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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