Document Detail


Kinetics of the enzyme-vesicle interaction including the formation of rafts and membrane strain.
MedLine Citation:
PMID:  22902353     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In cells, an appreciable part of enzymes is associated with lipid membranes. Academic experimental studies of the function of membrane enzymes (e.g., PLA(2) representing a prototype for interfacial enzymology) are often focused on the enzyme-vesicle interaction or, more specifically, on conversion of lipid forming the external leaflet of the vesicle membrane. The corresponding kinetics are complicated by many factors inherent to the interfacial physics and chemistry. The understanding of the relative role of such factors and how they should be quantitatively described is still limited. Here, we present the mean-field kinetic equations, taking the formation of rafts in the membrane and the product-induced membrane strain into account, and analyze various scenarios of lipid conversion. In particular, we scrutinize the conditions when the kinetics may exhibit a transition from a relatively long latency period to a steady-state regime with fast nearly constant reaction rate. Specifically, we discuss the likely role of the pore formation in the external lipid layer in this transition. The latter effect may be caused by the product-induced tensile strain in this layer.
Authors:
Vladimir P Zhdanov; Fredrik Höök
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-4
Journal Detail:
Title:  Biophysical chemistry     Volume:  170C     ISSN:  1873-4200     ISO Abbreviation:  Biophys. Chem.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-8-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  17-24     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Applied Physics, Chalmers University of Technology, S-41296 Göteborg, Sweden; Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia.
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