Document Detail


Kinetics of engraftment of CD34(-) and CD34(+) cells from mobilized blood differs from that of CD34(-) and CD34(+) cells from bone marrow.
MedLine Citation:
PMID:  11008020     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Mobilized peripheral blood (PB) progenitors are increasingly used in autologous and allogeneic transplantation. However, the short- and long-term engraftment potential of mobilized PB or bone marrow (BM) has not been directly compared. Although several studies showed that BM-derived Lin(-)CD34(-) cells contain hemopoietic progenitors, no studies have addressed whether Lin(-)CD34(-) cells from mobilized PB contain hemopoietic progenitors. Here, we compared the short- and long-term engraftment potential of CD34(+) cells and Lin(-)CD34(-) cells in BM and PB of normal donors who received 5 days of granulocyte colony-stimulating factor (G-CSF). MATERIALS AND METHODS: 35 x 10(3) CD34(+) or Lin(-)CD34(-) cells from G-CSF mobilized BM and PB of normal donors were transplanted in 60-day-old fetal sheep. Animals were evaluated 2 and 6 months after transplantation for human hemopoietic cells. In addition, cells recovered after 2 months from fetal sheep were serially passaged to secondary and tertiary recipients to assess long-term engrafting cells. RESULTS: Mobilized PB CD34(+) cells supported earlier development of human hemopoiesis than BM CD34(+) cells. When serially transferred to secondary and tertiary recipients, earlier exhaustion of human hematopoiesis was seen for PB than BM CD34(+) cells. A similar degree of chimerism was seen for Lin(-)CD34(-) cells from PB or BM in primary recipients. We again observed earlier exhaustion of human hemopoiesis with serial transplantation of PB than BM Lin(-)CD34(-) cells. CONCLUSIONS: Differences exist in the short- and long-term repopulating ability of cells in PB and BM from G-CSF mobilized normal donors, and this is independent of the phenotype. Studies are ongoing to examine if this reflects intrinsic differences in the repopulating potential between progenitors from PB and BM, or a lower frequency of long-term repopulating cells in PB than BM CD34(+) and Lin(-)CD34(-) cells, that may not be apparent if larger numbers of cells are transplanted.
Authors:
C M Verfaillie; G Almeida-Porada; S Wissink; E D Zanjani
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental hematology     Volume:  28     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-10-30     Completed Date:  2000-10-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1071-9     Citation Subset:  IM    
Affiliation:
Stem Cell Biology Program, Department of Medicine and Cancer Center, University of Minnesota Medical School, Minneapolis, Minn., USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / blood
Antigens, CD34 / blood*
Bone Marrow Cells / immunology
Bone Marrow Transplantation*
Cell Differentiation / drug effects
Cell Lineage / drug effects
Cell Transplantation / physiology
Fetus
Flow Cytometry
Hematopoiesis
Hematopoietic Stem Cell Mobilization*
Humans
Immunophenotyping
Kinetics
Sheep
Transplantation, Heterologous / methods
Grant Support
ID/Acronym/Agency:
HL-52955/HL/NHLBI NIH HHS; P01-CA-65493/CA/NCI NIH HHS; R01-DK-53673/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD34

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