Document Detail


Kinetics of bone marrow eosinophilopoiesis and associated cytokines after allergen inhalation.
MedLine Citation:
PMID:  14656753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Allergen inhalation is associated with increased eosinophil/basophil progenitors in bone marrow 24 hours after allergen inhalation. This study examined the kinetics of eosinophilopoiesis in dual (n = 14), compared with isolated early, responders (n = 12). Dual responders, in contrast to isolated early responders, develop significant sputum and blood eosinophilia and prolonged airway hyperresponsiveness. Bone marrow aspirates were taken before and 5, 12, 24, and 48 hours after allergen inhalation. In dual responders, increases in interleukin (IL)-3-responsive progenitors were detected as early as 5 hours after allergen inhalation, and IL-5-responsive progenitors were detected at 12 and 24 hours. No changes were detected in isolated early responders. Bone marrow IL-5 protein levels increased at 12 and 24 hours in dual responders only and these increases correlated with increases in IL-5-responsive progenitors. In addition, bone marrow IFN-gamma levels increased in dual responders at 48 hours. These data demonstrate that, in dual responders, there is allergen-induced activation of an eosinophilopoietic process that is rapid and sustained, and a relationship between increased bone marrow IL-5 levels and increased eosinophil production. We propose that after allergen inhalation, time-dependent changes in cytokine levels in the bone marrow control differentiation of eosinophil/basophil progenitors.
Authors:
Sandra C Dorman; Roma Sehmi; Gail M Gauvreau; Rick M Watson; Ronan Foley; Graham L Jones; Judah A Denburg; Mark D Inman; Paul M O'Byrne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-12-04
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  169     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-25     Completed Date:  2004-03-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  565-72     Citation Subset:  AIM; IM    
Affiliation:
Asthma Research Group, Firestone Institute for Respiratory Health, St. Joseph's Healthcare, Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adult
Allergens / adverse effects,  immunology
Asthma / blood,  complications,  immunology*
Bone Marrow / chemistry,  immunology*
Bronchial Hyperreactivity / complications,  immunology
Bronchial Provocation Tests
Cytokines / analysis,  blood,  immunology*
Eosinophils / immunology*
Female
Forced Expiratory Volume
Hematopoiesis / immunology*
Hematopoietic Stem Cells / immunology
Humans
Hypersensitivity, Immediate / complications
Inflammation
Interferon-gamma / immunology
Interleukin-10 / immunology
Interleukin-3 / immunology
Interleukin-5 / immunology
Leukocyte Count
Male
Middle Aged
Pulmonary Eosinophilia / etiology,  immunology*
Sputum / chemistry,  immunology
Time Factors
Up-Regulation / immunology
Chemical
Reg. No./Substance:
0/Allergens; 0/Cytokines; 0/Interleukin-3; 0/Interleukin-5; 130068-27-8/Interleukin-10; 82115-62-6/Interferon-gamma
Comments/Corrections
Comment In:
Am J Respir Crit Care Med. 2004 Mar 1;169(5):549-50   [PMID:  14982817 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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