Document Detail

Kinetics of binding properties of 5'-GMP with cisplatin under simulated physiological conditions by capillary electrophoresis.
MedLine Citation:
PMID:  10997716     Owner:  NLM     Status:  MEDLINE    
Kinetics of the adduct formation of cisplatin and its hydrolysis products with 5'-GMP was investigated using capillary electrophoresis (CE) under 'simulated physiological conditions'. Therefore, chloride ion and phosphate concentration as well as the pH were chosen comparable to intracellular conditions. Furthermore, the influence of the sulfur-containing alpha-amino acids L-methionine and L-cysteine on the kinetics of the adduct formation have been studied. Both amino acids increased the half-time of the reaction significantly but did not influence the formation of the major adduct cis-[Pt(NH3)2(N7-GMP)2]2-. The reaction products were separated using CE, and characterised using UV and NMR spectroscopy.
A Zenker; M Galanski; T L Bereuter; B K Keppler; W Lindner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  745     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-01-02     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  211-9     Citation Subset:  IM    
Institute of Inorganic Chemistry, University of Vienna, Austria.
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MeSH Terms
5'-Guanylic Acid / metabolism*
Chlorides / metabolism
Cisplatin / metabolism*
Cysteine / metabolism
Electrophoresis, Capillary / methods*
Magnetic Resonance Spectroscopy
Methionine / metabolism
Reg. No./Substance:
0/Chlorides; 15663-27-1/Cisplatin; 52-90-4/Cysteine; 63-68-3/Methionine; 85-32-5/5'-Guanylic Acid

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