| Kinetics of binding properties of 5'-GMP with cisplatin under simulated physiological conditions by capillary electrophoresis. | |
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MedLine Citation:
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PMID: 10997716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Kinetics of the adduct formation of cisplatin and its hydrolysis products with 5'-GMP was investigated using capillary electrophoresis (CE) under 'simulated physiological conditions'. Therefore, chloride ion and phosphate concentration as well as the pH were chosen comparable to intracellular conditions. Furthermore, the influence of the sulfur-containing alpha-amino acids L-methionine and L-cysteine on the kinetics of the adduct formation have been studied. Both amino acids increased the half-time of the reaction significantly but did not influence the formation of the major adduct cis-[Pt(NH3)2(N7-GMP)2]2-. The reaction products were separated using CE, and characterised using UV and NMR spectroscopy. |
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Authors:
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A Zenker; M Galanski; T L Bereuter; B K Keppler; W Lindner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 745 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2001-01-02 Completed Date: 2001-01-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 211-9 Citation Subset: IM |
Affiliation:
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Institute of Inorganic Chemistry, University of Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5'-Guanylic Acid
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metabolism* Chlorides / metabolism Cisplatin / metabolism* Cysteine / metabolism Electrophoresis, Capillary / methods* Kinetics Magnetic Resonance Spectroscopy Methionine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 15663-27-1/Cisplatin; 52-90-4/Cysteine; 63-68-3/Methionine; 85-32-5/5'-Guanylic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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