Document Detail


Kinetics of adsorption and desorption of aflatoxin B1 by viable and nonviable bacteria.
MedLine Citation:
PMID:  12636295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The reactions involved in the binding (adsorption) and release (desorption) of aflatoxin B1 (AFB1) to and from the surface of bacteria were investigated. Viable and heat-killed Lactobacillus rhamnosus GG, L. rhamnosus LC-705, and Propionibacterium freudenreichii subsp. shermanii JS were incubated in phosphate-buffered saline containing variable concentrations (0.0017 to 13.3 microg/ml) of AFB1. The relationship between the bacterial surface hydrophobicity and the AFB1 adsorption affinity was also investigated. A linear relationship was observed between the specific rate of AFB1 adsorption and the AFB1 concentration for all bacteria. The nature of desorption of adsorbed AFB1 was investigated by repetitive aqueous washes. A linear relationship was observed between the natural log value of the concentration of AFB1 adsorbed and the number of washes for all bacteria studied. The desorption constants were strain-dependent and were lower for heat-killed bacteria than for viable bacteria. Heat treatment appears to alter the surface properties of the bacteria rather than expose new adsorption sites. No correlation was found between the hydrophobicity and the AFB1 adsorption affinity.
Authors:
Y K Lee; H El-Nezami; C A Haskard; S Gratz; K Y Puong; S Salminen; H Mykkänen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  66     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-05-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  426-30     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore 117597.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Aflatoxin B1 / metabolism*
Chromatography, High Pressure Liquid
Food Handling / methods
Food Microbiology
Hot Temperature
Kinetics
Lactobacillus / metabolism*
Propionibacterium / metabolism*
Water / metabolism
Chemical
Reg. No./Substance:
1162-65-8/Aflatoxin B1; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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