| Kinetics of MHC-antigen complex formation on antigen-presenting cells. | |
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MedLine Citation:
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PMID: 2453565 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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With the use of flow cytometry, we recorded changes in intracellular ionized calcium [Ca2+]i of Indo-1 loaded T cells that were triggered by contact with APC. This rapid readout of TCR perturbation enabled us to monitor the formation of stimulatory Ag-MHC complexes on EBV-transformed B cells that were either pulsed with native tetanus toxoid (TT) or with a 12-amino-acid fragment of this protein. Neither unpulsed APC nor Ag-specific APC that were pulsed with native Ag and kept at +4 degrees C were able to induce changes in basal T cell [Ca2+]i in TT-specific T cell clones. After 1 h at 37 degrees C, however, the Ag-pulsed APC were able to induce a three-to-fourfold increase in [Ca2+]i. This length of time appeared to be almost independent of the concentration of Ag with which the APC were pulsed, suggesting that the lag time was due more to intracellular transit than to association of the processed Ag with the MHC molecule. Furthermore, the same lag time and independence of Ag concentration were found when the EBV-transformed B cells were pulsed with a mouse-anti-transferrin receptor mAb and tested for their capacity to trigger a T cell clone specific for processed mouse Ig. This indicates that, in addition to surface Ig, other receptors that are internalized can function in the same fashion in the uptake and processing of a soluble Ag. In contrast to what was found with intact native Ag, no lag time was observed when the APC were pulsed with high concentrations of a 12-amino-acid peptide, containing the amino acid sequence recognized by a TT-specific T cell clone, suggesting that the formation of MHC-peptide complexes occurs instantly. Pulsing with a lower peptide concentration, however, caused the appearance of a time-dependent increase in efficacy of Ag presentation, suggesting a slow accumulation of MHC-peptide complexes on the B cell membrane. |
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Authors:
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E Roosnek; S Demotz; G Corradin; A Lanzavecchia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 140 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1988 Jun |
Date Detail:
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Created Date: 1988-07-12 Completed Date: 1988-07-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4079-82 Citation Subset: AIM; IM |
Affiliation:
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Basel Institute for Immunology, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen-Presenting Cells / immunology, metabolism* Calcium / metabolism Clone Cells / immunology, metabolism Epitopes / immunology HLA-D Antigens / metabolism* Humans Intracellular Fluid / metabolism Kinetics Lymphocyte Activation Macromolecular Substances Mice Oligopeptides / immunology Receptors, Transferrin / physiology T-Lymphocytes / immunology, metabolism* Tetanus Toxoid / immunology Transferrin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/HLA-D Antigens; 0/Macromolecular Substances; 0/Oligopeptides; 0/Receptors, Transferrin; 0/Tetanus Toxoid; 11096-37-0/Transferrin; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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