| Kinetic study of the irreversible thermal and pressure inactivation of myrosinase from broccoli (Brassica oleracea L. Cv. italica). | |
| | |
MedLine Citation:
|
PMID: 10552454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Thermal and pressure inactivation of myrosinase from broccoli was kinetically investigated. Thermal inactivation proceeded in the temperature range 30-60 degrees C. These results indicate that myrosinase is rather thermolabile, as compared to other food quality related enzymes such as polyphenol oxidase, lipoxygenase, pectinmethylesterase, and peroxidase. In addition, a consecutive step model was shown to be efficient in modeling the inactivation curves. Two possible inactivation mechanisms corresponding to the consecutive step model were postulated. Pressure inactivation at 20 degrees C occurred at pressures between 200 and 450 MPa. In addition to its thermal sensitivity, the enzyme likewise is rather pressure sensitive as compared to the above-mentioned food quality related enzymes. By analogy with thermal inactivation, a consecutive step model could adequately describe pressure inactivation curves. At 35 degrees C, pressure inactivation was studied in the range between 0. 1 and 450 MPa. Application of low pressure (<350 MPa) resulted in retardation of thermal inactivation, indicating an antagonistic or protective effect of low pressure. |
| | |
Authors:
|
L Ludikhuyze; V Ooms; C Weemaes; M Hendrickx |
Related Documents
:
|
19954404 - Improving rheological characteristics of hydrophilic ointment base by treatment with a ... 11805314 - Redefinition of the mode grüneisen parameter for polyatomic substances and thermodynam... 6301124 - Patterns of interaction of effects of light metabolically inert gases with those of hyd... 19895024 - In situ high-pressure study of diborane by infrared spectroscopy. 6104264 - Volume of the ventricles in benign intracranial hypertension. 14581294 - Adiposity is related to gender differences in impaired stress-induced pressure natriure... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: 47 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 1999 May |
Date Detail:
|
Created Date: 2000-08-18 Completed Date: 2000-08-18 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1794-800 Citation Subset: IM |
Affiliation:
|
Department of Food and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Brassica
/
enzymology* Enzyme Stability Glycoside Hydrolases / antagonists & inhibitors, chemistry* Hot Temperature Kinetics Pressure Thermodynamics |
| Chemical | |
Reg. No./Substance:
|
EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.147/thioglucosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nonenzymatic browning reaction of essential amino acids: effect of pH on caramelization and Maillard...
Next Document: Evening primrose meal: a source of natural antioxidants and scavenger of hydrogen peroxide and oxyge...