Document Detail


Kinetic studies of recombinant rabies virus glycoprotein (RVGP) cDNA transcription and mRNA translation in Drosophila melanogaster S2 cell populations.
MedLine Citation:
PMID:  23340966     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Recombinant rabies virus glycoprotein (RVGP) was expressed in cell membranes of stably transfected Drosophila S2 cells using constitutive and inducible promoters. Although with quantitative differences of RVGP expression in both systems, the cDNA transcription, as evaluated by relative RVGP mRNA levels measured by qRT-PCR, sustained the amount of RVGP producing cells and the RVGP volumetric (ΠRVGP) productivity. At the transition to the stationary cell growth phase, once the cell culture slowed down its rate of multiplication, an accumulation of RVGP mRNA and RVGP was clearly observed in both cell populations. Nevertheless, cell cultures performed under sub-optimal temperatures indicated that an envisaged increase in the RVGP production is not only dependent on cell growth rate, but essentially on optimal cell metabolic state.
Authors:
R M Astray; S A C Jorge; M A N Lemos; A Y Yokomizo; V L L Boldorini; A L P Puglia; O G Ribeiro; C A Pereira
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Publication Detail:
Type:  Journal Article     Date:  2013-01-23
Journal Detail:
Title:  Cytotechnology     Volume:  65     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-25     Completed Date:  2013-10-25     Revised Date:  2014-03-28    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  829-38     Citation Subset:  -    
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