| Kinetic studies of recombinant rabies virus glycoprotein (RVGP) cDNA transcription and mRNA translation in Drosophila melanogaster S2 cell populations. | |
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MedLine Citation:
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PMID: 23340966 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Recombinant rabies virus glycoprotein (RVGP) was expressed in cell membranes of stably transfected Drosophila S2 cells using constitutive and inducible promoters. Although with quantitative differences of RVGP expression in both systems, the cDNA transcription, as evaluated by relative RVGP mRNA levels measured by qRT-PCR, sustained the amount of RVGP producing cells and the RVGP volumetric (Π(RVGP)) productivity. At the transition to the stationary cell growth phase, once the cell culture slowed down its rate of multiplication, an accumulation of RVGP mRNA and RVGP was clearly observed in both cell populations. Nevertheless, cell cultures performed under sub-optimal temperatures indicated that an envisaged increase in the RVGP production is not only dependent on cell growth rate, but essentially on optimal cell metabolic state. |
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Authors:
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R M Astray; S A C Jorge; M A N Lemos; A Y Yokomizo; V L L Boldorini; A L P Puglia; O G Ribeiro; C A Pereira |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-23 |
Journal Detail:
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Title: Cytotechnology Volume: - ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-900, Brazil, renatoastray@butantan.gov.br. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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