| KINETIC STUDIES OF L-ASPARAGINASE FROM Penicillium digitatum. | |
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MedLine Citation:
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PMID: 23030468 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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L-Asparaginase is an enzyme used in the treatment of acute lymphoblastic leukemia and other related malignancies. Its further use includes reduction of asparagine concentration in food products, which may lead to formation of acrylamide. Currently bacterial asparaginase is produced at industrial scale, but the enzyme isolated from bacterial origin is often associated with adverse reactions. These side effects require development of asparaginase from alternative sources. In the present study, Penicillium digitatum was explored for the production of extracellular L-asparaginase using modified Czapek-Dox media. The enzyme was purified about 60.95-fold and then kinetic study showed that the Km value of the enzyme was 1 × 10(-5) M. The optimum pH and temperature for the enzyme were 7.0 and 30°C, respectively. The optimum incubation period for L-asparaginase was 15 min. This work concludes that this enzyme can be a suitable candidate due to its strong kinetic properties, and further research can usher into development of asparaginase formulation from fungal origin with less adverse effects. |
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Authors:
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Abhinav Shrivastava; Abdul Arif Khan; Archana Shrivastav; Sudhir K Jain; Pradeep K Singhal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Preparative biochemistry & biotechnology Volume: 42 ISSN: 1532-2297 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 574-81 Citation Subset: IM |
Affiliation:
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a College of Life Sciences, Cancer Hospital & Research Institute , Gwalior (MP) , India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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