Document Detail


Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane.
MedLine Citation:
PMID:  8161167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bacteriocin lactacin F is bactericidal against Lactobacillus delbrueckii, Lactobacillus helveticus, and Enterococcus faecalis. Activity against L. delbrueckii was recently shown to be dependent on two peptides, LafA and LafX, which are encoded within the lactacin F operon (T. R. Klaenhammer, FEMS Microbiol. Rev. 12:39-87, 1993). It has been proposed that two peptides form an active lactacin F complex. In this study, the action of lactacin F against E. faecalis ATCC 19443 and the effects of various environmental parameters were investigated in detail. Addition of lactacin F induced the loss of K+ from cells of L. delbrueckii, Lactobacillus johnsonii 88-4, and E. faecalis, while the lactacin F producer L. johnsonii VPI 11088 was not affected by the bacteriocin. Lactacin F caused an immediate loss of cellular K+, depolarization of the cytoplasmic membrane, and hydrolysis of internal ATP in E. faecalis. Lactacin F induced loss of K+ in 3,3',4',5-tetrachlorosalicylanilide-treated cells, indicating that pores are formed in the absence of a proton motive force. ATP hydrolysis was not due to dissipation of the proton motive force but was most likely caused by efflux of inorganic phosphate, resulting in a shift of the ATP hydrolysis equilibrium. Action of lactacin F was optimal at acidic pH values and was reduced in the presence of di- and trivalent cations. The lanthanide gadolinium (Gd3+) prevented action of lactacin F completely at a concentration of 0.2 mM. Lactacin F-induced loss of cell K+ was severely reduced at low temperatures, presumably as a result of increased ordering of the lipid hydrocarbon chains in the cytoplasmic membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
T Abee; T R Klaenhammer; L Letellier
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  60     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-05-13     Completed Date:  1994-05-13     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1006-13     Citation Subset:  IM    
Affiliation:
Department of Food Science, Agricultural University Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Bacteriocins / pharmacology*
Cell Membrane / drug effects,  metabolism
Detergents / pharmacology
Enterococcus faecalis / drug effects,  metabolism*
Hydrogen-Ion Concentration
Lactobacillus / drug effects,  metabolism*
Membrane Potentials
Potassium / metabolism*
Temperature
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/Detergents; 107950-55-0/lactacin F; 56-65-5/Adenosine Triphosphate; 7440-09-7/Potassium
Comments/Corrections

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