Document Detail


Kinetic and steady-state investigations of solute accumulation in bacterial membranes by continuously monitoring the radioactivity in the effluent of flow-dialysis experiments.
MedLine Citation:
PMID:  6967404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The flow-dialysis technique for studies of solute accumulation by membrane preparations has been made more suitable for routine measurements by recording continuously the radioactivity in the effluent of a flow-dialysis vessel with a homogeneous flow-monitoring device for beta-emitters. This modification not only decreases the time and cost of a flow-dialysis experiment but also allows the investigator to react directly on the outcome of his experiments. Analysis of the kinetics of this automated flow-dialysis system shows that this technique can also be used for the determination of the rate of uptake of solutes into bacterial membranes. This has been confirmed in Rhodopseudomonas sphaeroides by comparing the results of Rb+ and inorganic phosphate uptake studies performed by automated flow-dialysis and by the conventional filtration procedure. This application has the limitation that solute uptake has to proceed linearly for a period of about five times the half-time of the response of the flow-dialysis system. The two described applications make automated flow-dialysis very well-suited for experiments on the bioenergetics and regulation of solute uptake into bacterial membranes. Both driving force and rate of solute uptake can now be determined in one experiment.
Authors:
K J Hellingwerf; W N Konings
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  106     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1980 May 
Date Detail:
Created Date:  1980-10-24     Completed Date:  1980-10-24     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  431-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / metabolism*
Dialysis / methods*
Kinetics
Phosphates / metabolism
Rhodobacter sphaeroides / metabolism
Rubidium / metabolism
Chemical
Reg. No./Substance:
0/Phosphates; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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