Document Detail


Kinetic spectrofluorimetric determination of trace ascorbic acid based on its inhibition on the oxidation of pyronine Y by nitrite.
MedLine Citation:
PMID:  15683787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly sensitive spectrofluorimetric method is proposed for the determination of trace amount of ascorbic acid using a new indication. The method is based on the inhibition of ascorbic acid on the oxidation of pyronine Y (PRY) by nitrite. The detection limit for ascorbic acid is 0.012 microg ml(-1), the linear range of the determination is 0.02-0.36 microg ml(-1). Analytical parameters, such as reagent concentration, pH, reaction temperature and time, were optimized. The relative standard deviations of eleven replication determinations of 0.12 and 0.24 microg ml(-1) ascorbic acid were 1.4 and 0.72%, respectively. This method has been used to determine ascorbic acid in pharmaceuticals, vegetables, fruits and soft drink with satisfactory results.
Authors:
Suling Feng; Jin Wang; Xingguo Chen; Jing Fan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy     Volume:  61     ISSN:  1386-1425     ISO Abbreviation:  Spectrochim Acta A Mol Biomol Spectrosc     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-01     Completed Date:  2005-03-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9602533     Medline TA:  Spectrochim Acta A Mol Biomol Spectrosc     Country:  England    
Other Details:
Languages:  eng     Pagination:  841-4     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
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MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / analysis*,  metabolism
Nitrites / metabolism*
Pyronine / metabolism*
Spectrometry, Fluorescence
Sulfuric Acids / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Nitrites; 0/Sulfuric Acids; 50-81-7/Ascorbic Acid; 7664-93-9/sulfuric acid; 92-32-0/Pyronine

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