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Kinetic relations of the Na-amino acid interaction at the mucosal border of intestine.
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MedLine Citation:
PMID:  6033585     Owner:  NLM     Status:  MEDLINE    
The relation between unidirectional influxes of Na and amino acids across the mucosal border of rabbit ileum was studied under a variety of conditions. At constant Na concentration in the mucosal bathing solution, amino acid influx followed Michaelis-Menten kinetics permitting determination of maximal influx and the apparent Michaelis constant, K(t). Reduction in Na concentration, using choline as substitute cation, caused an increase in K(t) for alanine but had no effect on maximal alanine influx. The reciprocal of K(t) was a linear function of Na concentration. Similar results were obtained for valine and leucine and these amino acids competitively inhibited alanine influx both in the presence and in the absence of Na. These results lead to a model for the transport system which involves combination of Na and amino acid with a single carrier or site leading to penetration of both solutes. The model predicts that alanine should cause an increase in Na influx and the ratio of this extra Na flux to alanine flux should vary with Na concentration. The observed relation agreed closely with predicted values for Na concentrations from 5 to 140 mM. These results support the hypothesis that interactions between Na and amino acid transport depend in part on a common entry mechanism at the mucosal border of the intestine.
P F Curran; S G Schultz; R A Chez; R E Fuisz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  50     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1967 May 
Date Detail:
Created Date:  1967-10-07     Completed Date:  1967-10-07     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1261-86     Citation Subset:  IM    
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MeSH Terms
Alanine / metabolism
Amino Acids / metabolism*
Biological Transport, Active
Cell Membrane Permeability
Choline / pharmacology
Ileum / metabolism
Intestinal Mucosa / metabolism*
Leucine / metabolism
Models, Theoretical
Sodium / metabolism*
Valine / metabolism
Reg. No./Substance:
0/Amino Acids; 56-41-7/Alanine; 61-90-5/Leucine; 62-49-7/Choline; 7004-03-7/Valine; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1967 by The Rockefeller University Press
Received Day: 6 Month: 7 Year: 1966
Print publication date: Day: 1 Month: 5 Year: 1967
Volume: 50 Issue: 5
First Page: 1261 Last Page: 1286
ID: 2225714
PubMed Id: 6033585

Kinetic Relations of the Na-Amino Acid Interaction at the Mucosal Border of Intestine
Peter F. Curran
Stanley G. Schultz
Ronald A. Chez
Robert E. Fuisz
From the Biophysical Laboratory, Harvard Medical School, Boston, Massachusetts.
Dr. Chez's present address is the University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania. Dr. Fuisz's present address is the Peter Bent Brigham Hospital, Boston, Massachusetts

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