| Kinetic and pharmacological properties of [(3)H]-histamine transport into cultured type 1 astrocytes from neonatal rats. | |
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MedLine Citation:
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PMID: 19184360 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE AND DESIGN: Astrocytes actively participate in the inactivation of neurotransmitters. In this work we elucidated the contribution of astrocytes in clearance of histamine, a process which has not yet been fully clarified. METHODS: The characteristics of [(3)H]-histamine uptake were determined in cultured neonatal rat type 1 astrocytes and histamine-N-methyl-transferase expression was determined using RT-PCR. RESULTS: These cells transport [(3)H]-histamine in a time- and concentration-dependent manner. The histamine clearance by astrocytes was described by a mathematical model including two processes: electrodiffusion and active transport. A further analysis of kinetic parameters of a carrier-operated transport revealed a single transport system with Michaelis constant (K(m)) of 3.5 +/- 0.8 microM and a maximal uptake rate (V(max)) of 7.9 +/- 0.3 pmol/mg protein/min. From drugs tested amitriptyline, desipramine, mepyramine and cimetidine significantly decreased [(3)H]-histamine uptake. Taken-up histamine could be metabolically degraded in cultured astrocytes, since they express mRNA for enzyme histamine-N-methyltransferase. CONCLUSIONS: Astrocytes participate in the clearance of extracellular histamine by electrodiffusion and active transport by a yet not identified carrier. Taken up histamine can be converted to tele-methylhistamine within astrocytes thus indicating the involvement of astrocytes not only in clearance but also in the inactivation of histamine. |
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Authors:
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D Osredkar; T Burnik-Papler; B Pecavar; V Kralj-Iglic; M Krzan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Inflammation research : official journal of the European Histamine Research Society ... [et al.] Volume: 58 ISSN: 1420-908X ISO Abbreviation: Inflamm. Res. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-02-02 Completed Date: 2009-04-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508160 Medline TA: Inflamm Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 94-102 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Antidepressive Agents / pharmacology Astrocytes / cytology, metabolism* Biological Transport / drug effects, physiology Cell Membrane / metabolism Cells, Cultured Histamine / chemistry, metabolism* Histamine Antagonists / metabolism Histamine N-Methyltransferase / metabolism Models, Theoretical Rats Receptors, Histamine / metabolism Tritium / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antidepressive Agents; 0/Histamine Antagonists; 0/Receptors, Histamine; 10028-17-8/Tritium; 51-45-6/Histamine; EC 2.1.1.8/Histamine N-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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