Document Detail


Kinetic method for the determination of traces of thyroxine by its catalytic effect on the Mn(III) metaphosphate-As(III) reaction.
MedLine Citation:
PMID:  18371817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new, highly sensitive and simple kinetic method for the determination of thyroxine was proposed. The method was based on the catalytic effect of thyroxine on the oxidation of As(III) by Mn(III) metaphosphate. The kinetics of the reaction was studied in the presence of orthophosphoric acid. The reaction rate was followed spectrophotometrically at 516 nm. It was established that orthophosphoric acid increased the reaction rate and that the extent of the non-catalytic reaction was extremely small. A kinetic equation was postulated and the apparent rate constant was calculated. The dependence of the reaction rate on temperature was investigated and the energy of activation and other kinetic parameters were determined. Thyroxine was determined under the optimal experimental conditions in the range 7.0 x 10(-9) to 3.0 x 10(-8) mol L(-1) with a relative standard deviation up to 6.7% and a detection limit of 2.7 x 10(-9) molL(-1). In the presence of 0.08 mol L(-1) chloride, the detection limit decreased to 6.6 x 10(-10) mol L(-1). The proposed method was applied for the determination of thyroxine in tablets. The accuracy of the method was evaluated by comparison with the HPLC method.
Authors:
Ferenc T Pastor; Gordana A Milovanović; Marija Todorović
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-02
Journal Detail:
Title:  Talanta     Volume:  74     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-03-28     Completed Date:  2008-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1556-61     Citation Subset:  IM    
Affiliation:
Faculty of Chemistry, University of Belgrade, P.O. Box 158, 11001 Belgrade, Studentski trg 12-16, Serbia.
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MeSH Terms
Descriptor/Qualifier:
Arsenic / chemistry
Catalysis
Kinetics
Manganese / chemistry
Phosphoric Acids
Phosphorous Acids / chemistry
Spectrum Analysis
Tablets / analysis
Temperature
Thyroxine / analysis*
Chemical
Reg. No./Substance:
0/Phosphoric Acids; 0/Phosphorous Acids; 0/Tablets; 10343-62-1/metaphosphoric acid; 7439-96-5/Manganese; 7440-38-2/Arsenic; 7488-70-2/Thyroxine; 7664-38-2/phosphoric acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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