| Kinetic mechanism of non-muscle myosin IIB: functional adaptations for tension generation and maintenance. | |
| | |
MedLine Citation:
|
PMID: 12704189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Besides driving contraction of various types of muscle tissue, conventional (class II) myosins serve essential cellular functions and are ubiquitously expressed in eukaryotic cells. Three different isoforms in the human myosin complement have been identified as non-muscle class II myosins. Here we report the kinetic characterization of a human non-muscle myosin IIB subfragment-1 construct produced in the baculovirus expression system. Transient kinetic data show that most steps of the actomyosin ATPase cycle are slowed down compared with other class II myosins. The ADP affinity of subfragment-1 is unusually high even in the presence of actin filaments, and the rate of ADP release is close to the steady-state ATPase rate. Thus, non-muscle myosin IIB subfragment-1 spends a significantly higher proportion of its kinetic cycle strongly attached to actin than do the muscle myosins. This feature is even more pronounced at slightly elevated ADP levels, and it may be important in carrying out the cellular functions of this isoform working in small filamentous assemblies. |
| | |
Authors:
|
Fei Wang; Mihaly Kovacs; Aihua Hu; John Limouze; Estelle V Harvey; James R Sellers |
Related Documents
:
|
1755369 - X-ray diffraction studies on muscle regulation. 10497209 - Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin ... 2752729 - Effect of the androgenic status on the phenotype of the plantaris muscle of the rat. 12673599 - Skeletal muscle myosin cross-bridge cycling is necessary for myofibrillogenesis. 3730499 - "crystalline" myosin cross-bridge array in relaxed bony fish muscle. low-angle x-ray di... 7190649 - Fraying of a-filaments into three subfilaments. 22174829 - Smyd1b_tv1, a key regulator of sarcomere assembly, is localized on the m-line of skelet... 3207169 - Back muscle function during bipedal walking in chimpanzee and gibbon: implications for ... 11316639 - Effects of emphysema on diaphragm microvascular oxygen pressure. |
Publication Detail:
|
Type: Journal Article Date: 2003-04-17 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 278 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2003 Jul |
Date Detail:
|
Created Date: 2003-07-21 Completed Date: 2003-08-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 27439-48 Citation Subset: IM |
Affiliation:
|
Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892-1762, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actins
/
chemistry Actomyosin / chemistry Adenosine Diphosphate / chemistry Adenosine Triphosphatases / chemistry Adenosine Triphosphate / chemistry, metabolism, pharmacology Animals Cloning, Molecular DNA, Complementary / metabolism Dose-Response Relationship, Drug Humans Hydrolysis Kinetics Models, Chemical Nonmuscle Myosin Type IIB / chemistry* Phosphorylation Protein Isoforms Pyrenes / chemistry Rabbits Thermodynamics Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 0/DNA, Complementary; 0/Protein Isoforms; 0/Pyrenes; 129-00-0/pyrene; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 9013-26-7/Actomyosin; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/Nonmuscle Myosin Type IIB |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: GATA-4 is a nuclear mediator of mechanical stretch-activated hypertrophic program.
Next Document: The F-actin cross-linking and focal adhesion protein filamin A is a ligand and in vivo substrate for...