Document Detail

Kinetic capillary electrophoresis (KCE): a conceptual platform for kinetic homogeneous affinity methods.
MedLine Citation:
PMID:  16316258     Owner:  NLM     Status:  MEDLINE    
We propose kinetic capillary electrophoresis (KCE) as a conceptual platform for the development of kinetic homogeneous affinity methods. KCE is defined as the CE separation of species that interact during electrophoresis. Depending on how the interaction is arranged, different KCE methods can be designed. All KCE methods are described by the same mathematics: the same system of partial differential equations with only initial and boundary conditions being different. Every qualitatively unique set of initial and boundary conditions defines a unique KCE method. Here, we (i) present the theoretical bases of KCE, (ii) define four new KCE methods, and (iii) propose a multimethod KCE toolbox as an integrated kinetic technique. Using the KCE toolbox, we were able to, for the first time, observe high-affinity (specific) and low-affinity (nonspecific) interactions within the same protein-ligand pair. The concept of KCE allows for the creation of an expanding toolset of powerful kinetic homogeneous affinity methods, which will find their applications in studies of biomolecular interactions, quantitative analyses, and selecting affinity probes and drug candidates from complex mixtures.
Alexander Petrov; Victor Okhonin; Maxim Berezovski; Sergey N Krylov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  127     ISSN:  0002-7863     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-30     Completed Date:  2006-02-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17104-10     Citation Subset:  IM    
Department of Chemistry, York University, Toronto, Ontario, Canada.
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MeSH Terms
DNA, Single-Stranded / chemistry
DNA-Binding Proteins / chemistry
Electrophoresis, Capillary / methods*
Models, Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; 0/DNA-Binding Proteins

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