Document Detail


Kinetic analysis of growth rate, ATP, and pigmentation suggests an energy-spilling function for the pigment prodigiosin of Serratia marcescens.
MedLine Citation:
PMID:  18805986     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serratia marcescens is a gram-negative environmental bacterium and opportunistic pathogen. S. marcescens expresses prodigiosin, a bright red and cell-associated pigment which has no known biological function for producing cells. We present here a kinetic model relating cell, ATP, and prodigiosin concentration changes for S. marcescens during cultivation in batch culture. Cells were grown in a variety of complex broth media at temperatures which either promoted or essentially prevented pigmentation. High growth rates were accompanied by large decreases in cellular prodigiosin concentration; low growth rates were associated with rapid pigmentation. Prodigiosin was induced most strongly during limited growth as the population transitioned to stationary phase, suggesting a negative effect of this pigment on biomass production. Mathematically, the combined rate of formation of biomass and bioenergy (as ATP) was shown to be equivalent to the rate of prodigiosin production. Studies with cyanide inhibition of both oxidative phosphorylation and pigment production indicated that rates of biomass and net ATP synthesis were actually higher in the presence of cyanide, further suggesting a negative regulatory role for prodigiosin in cell and energy production under aerobic growth conditions. Considered in the context of the literature, these results suggest that prodigiosin reduces ATP production by a process termed energy spilling. This process may protect the cell by limiting production of reactive oxygen compounds. Other possible functions for prodigiosin as a mediator of cell death at population stationary phase are discussed.
Authors:
Pryce L Haddix; Sarah Jones; Pratik Patel; Sarah Burnham; Kaori Knights; Joan N Powell; Amber LaForm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-19
Journal Detail:
Title:  Journal of bacteriology     Volume:  190     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2009-01-13     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7453-63     Citation Subset:  IM    
Affiliation:
Department of Biology, Auburn University Montgomery, P.O. Box 244023, Montgomery, AL 36124-4023, USA. phaddix@aum.edu
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism*
Culture Media / pharmacology
Cyanides / pharmacology
Inositol / pharmacology
Kinetics
Oxidative Phosphorylation / drug effects
Pigmentation / physiology
Prodigiosin / biosynthesis*
Serratia marcescens / drug effects,  growth & development*,  metabolism*
Temperature
Chemical
Reg. No./Substance:
0/Culture Media; 0/Cyanides; 56-65-5/Adenosine Triphosphate; 6917-35-7/Inositol; 82-89-3/Prodigiosin
Comments/Corrections

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