| Killing of Treponema denticola by mouse peritoneal macrophages. | |
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MedLine Citation:
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PMID: 20200417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Treponema denticola has been identified as an important cause of periodontal disease and hypothesized to be involved in extra-oral infections. The objective of this study was to investigate the role of T. denticola cell length and motility during mouse peritoneal macrophages in vitro uptake. Macrophages, incubated under aerobic and anaerobic conditions, produced a similar amount of TNF-alpha when stimulated with Escherichia coli LPS. The uptake of FlgE- and CfpA-deficient mutants of T. denticola was significantly increased compared with the wild-type strain, due to cell size or lack of motility. Opsonization with specific antibodies considerably improved the treponemes' uptake. These results suggest that macrophages, in addition to other phagocytes, could play an important role in the control of T. denticola infection, and that the raising of specific antibodies could improve the efficacy of the immune response toward T. denticola, either at an oral site or during dissemination. |
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Authors:
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P Gaibani; C Vocale; S Ambretti; F Cavrini; J Izard; L Miragliotta; M T Pellegrino; V Sambri |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-03-03 |
Journal Detail:
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Title: Journal of dental research Volume: 89 ISSN: 1544-0591 ISO Abbreviation: J. Dent. Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-16 Completed Date: 2010-05-04 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: United States |
Other Details:
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Languages: eng Pagination: 521-6 Citation Subset: D; IM |
Affiliation:
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Department of Haematology and Oncology L. and A. Seragnoli, Section of Microbiology, University of Bologna, St. Orsola Hospital, 9 via G. Massarenti, 40138 Bologna, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Anaerobiosis Animals Antibodies, Bacterial / immunology Bacterial Proteins / genetics, immunology Cells, Cultured Cytoskeletal Proteins / genetics Escherichia coli Fluorescent Antibody Technique, Indirect Immunoglobulin E / genetics Lipopolysaccharides / pharmacology Macrophages, Peritoneal / drug effects, immunology* Membrane Proteins / immunology Mice Mutation / genetics Phagocytosis / immunology* Treponema denticola / genetics, immunology* Tumor Necrosis Factor-alpha / analysis |
| Grant Support | |
ID/Acronym/Agency:
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DE017106/DE/NIDCR NIH HHS; R21 DE017106-01A1/DE/NIDCR NIH HHS; R21 DE017106-02/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Bacterial Proteins; 0/Cytoskeletal Proteins; 0/Lipopolysaccharides; 0/Membrane Proteins; 0/Tumor Necrosis Factor-alpha; 37341-29-0/Immunoglobulin E |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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