Document Detail

Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum-sensing system.
MedLine Citation:
PMID:  12713492     Owner:  NLM     Status:  MEDLINE    
Burkholderia cepacia H111, which was isolated from a cystic fibrosis patient, effectively kills the nematode Caenorhabditis elegans. Depending on the medium used for growth of the bacterium two different killing modes were observed. On high-osmolarity medium the nematodes became paralysed and died within 24 h. Using filter assays we provide evidence that this killing mode involves the production of an extracellular toxin. On nematode growth medium killing occurs over the course of 2-3 days and involves the accumulation of bacteria in the intestinal lumen of C. elegans. We demonstrate that the cep quorum-sensing system of H111 is required for efficient killing of C. elegans under both killing conditions. Using the C. elegans phm-2 mutant that has a non-functional grinder evidence is provided that the cep system is required to enter the intestinal lumen but is dispensable for the colonization of the gut. Furthermore, we demonstrate that the type II secretion machinery is not essential for nematode killing.
Manuela Köthe; Melanie Antl; Birgit Huber; Kilian Stoecker; Doreen Ebrecht; Ivo Steinmetz; Leo Eberl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular microbiology     Volume:  5     ISSN:  1462-5814     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-25     Completed Date:  2003-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  343-51     Citation Subset:  IM    
Lehrstuhl für Mikrobiologie, Technische Universität München, Am Hochanger 4, 85350 Freising, Germany.
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MeSH Terms
Bacterial Proteins / genetics,  physiology
Burkholderia cepacia / genetics,  pathogenicity*,  physiology
Caenorhabditis elegans / genetics,  microbiology*
Caenorhabditis elegans Proteins / genetics
Genes, Bacterial
Genes, Helminth
Intestines / microbiology
Virulence / genetics,  physiology
Reg. No./Substance:
0/Bacterial Proteins; 0/Caenorhabditis elegans Proteins; 0/CepR protein, Burkholderia; 0/phm-2 protein, C elegans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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