Document Detail

Killed Bacillus subtilis spores expressing streptavidin: a novel carrier of drugs to target cancer cells.
MedLine Citation:
PMID:  23480726     Owner:  NLM     Status:  Publisher    
Abstract Carriers of drugs in cancer therapy are required to reduce side-effects of the drugs to normal cells. Here we constructed killed recombinant Bacillus subtilis spores (SA1) that expressed streptavidin as a chimeric fusion to the spore coat protein CotB and used the spores as bioparticle carrier. When bound with biotinylated cetuximab these spores could specifically target to the epidermal growth factor receptor on HT 29 colon cancer cells, thereby delivered paclitaxel to the cells with 4-fold higher efficiency, as indicated by fluorescent intensity of paclitaxel Oregon Green 488 bound to HT29 cells. Based on real-time monitoring of cell index, the IC50 of growth of HT29 cells by paclitaxel-SA1-cetuximab was estimated to be 2.9 nM approximately 5-fold lower than water-soluble paclitaxel (14.5 nM). Instability of DNA content was observed when cells were treated with 16 nM paclitaxel-SA1-cetuximab, resulting in a 2-fold enhancement in polyploidy cells. Thus, by targeting the release of paclitaxel to HT29 cells, spore-associated cetuximab augmented the inhibitory effect of paclitaxel on cell division and proliferation. The SA1 could be used as a "universal" drug carrier to target specific biomarkers on cancer cells by conjugating with suitable biotinylated antibodies.
Van Anh Thi Nguyen; Hong Anh Huynh; Tong Van Hoang; Ngoc Thi Ninh; An Thi Hong Pham; Hoa Anh Nguyen; Tuan-Nghia Phan; Simon M Cutting
Related Documents :
24525066 - The impacts of replacing air bubbles with microspheres for the clarification of algae f...
23900306 - Antiproliferation and anti-migration induced by gypenosides in human colon cancer sw620...
23650256 - N-glycosylation of gel1 or gel2 is vital for cell wall β-glucan synthesis in aspergillu...
1164126 - Plasma cells in urine: occurrence in multiple myeloma.
10427136 - Ceramide toxicity and metabolism differ in wild-type and multidrug-resistant cancer cells.
1584806 - Primary structure and expression of a gamete lytic enzyme in chlamydomonas reinhardtii:...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-12
Journal Detail:
Title:  Journal of drug targeting     Volume:  -     ISSN:  1029-2330     ISO Abbreviation:  J Drug Target     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312476     Medline TA:  J Drug Target     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Key laboratory of Enzyme and Protein Technology, VNU University of Science , Hanoi , Vietnam .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Role of glucose transporters in the intestinal absorption of gastrodin, a highly water-soluble drug ...
Next Document:  Use of airway pressure release ventilation in a child with refractory hepatopulmonary syndrome after...