Document Detail

Kidney tubular epithelium cells and vascular smooth muscle cells contain different types of atrial natriuretic factor receptors.
MedLine Citation:
PMID:  2853738     Owner:  NLM     Status:  MEDLINE    
We have identified and characterized three distinct atrial natriuretic factor (ANF) receptor subtypes from cultured canine kidney tubular (MDCK) cells and rat thoracic aortic smooth muscle cells. These are (1) a disulphide-linked 140-kDa protein found in rat thoracic aortic smooth muscle cells which was reduced by dithiothreitol to a 70-kDa band, (2) a disulphide-unlinked 120-kDa protein, specific to canine kidney tubular cells, whose molecular weight was not reduced by dithiothreitol and (3) a 66-70 kDa protein prevalent in both types of cell whose molecular weight was not reduced by dithiothreitol. The non-reducible 66-70 kDa and the reducible 140 kDa proteins showed strong affinities to the full-length ANF-(99-126) and truncated ANF-(103-123); however, the non-reducible 120-kDa protein showed strong affinity only to ANF-(99-126). Both ANF-(99-126) and ANF-(103-123) stimulated cyclic (c)GMP in the rat smooth muscle cells but only ANF-(99-126) stimulated cGMP in the canine tubular cells. Distinct ANF receptor subtypes might be linked to diverse physiological functions of ANF such as natriuresis and diuresis in kidney and vasorelaxation in vascular smooth muscle cells.
K N Pandey; T Inagami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of hypertension. Supplement : official journal of the International Society of Hypertension     Volume:  6     ISSN:  0952-1178     ISO Abbreviation:  J Hypertens Suppl     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-05-18     Completed Date:  1989-05-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8501422     Medline TA:  J Hypertens Suppl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  S292-4     Citation Subset:  IM    
Department of Biochemistry, Vanderbilt University, School of Medicine, Nashville, TN 37232.
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MeSH Terms
Affinity Labels
Cyclic AMP / biosynthesis
Epithelium / metabolism
Kidney Tubules / metabolism*
Molecular Weight
Muscle, Smooth, Vascular / metabolism*
Receptors, Atrial Natriuretic Factor
Receptors, Cell Surface / classification,  metabolism*
Grant Support
Reg. No./Substance:
0/Affinity Labels; 0/Disulfides; 0/Receptors, Cell Surface; 60-92-4/Cyclic AMP; EC, Atrial Natriuretic Factor

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