Document Detail

Kidney proximal tubule cells: epithelial cells without EGTA-extractable annexins?
MedLine Citation:
PMID:  11012089     Owner:  NLM     Status:  MEDLINE    
The expression and the subcellular localizations of annexins I, II, IV, VI, and XIII in renal epithelial cells were investigated, using immunological techniques with specific monoclonal antibodies. Upon performing Western blotting experiments, no annexins VI and XIII were detected in kidney, whereas annexins I, II, and IV were. Immunofluorescence labelling procedure performed on thin frozen renal sections showed the presence of these three annexins along the plasma membrane of the collecting duct cells with a restricted expression of annexin I at principal cells. Annexin I was also found present in some glomerular cells. None of these annexins, however, were detected in the proximal tubular cells upon performing immunofluorescence labelling and electrophoretic analysis on an EGTA (ethylenebis(oxyethylenenitrilo)tetraacetic acid)-extractable annexin fraction prepared from freshly isolated cells. This is the first time a mammalian epithelial cell has been found to express non-typical annexin (at least partly solubilized with EGTA). However, when these cells were grown in primary culture, they were found to express annexins I, II, IV, and V. As well as being located along the basolateral membrane, annexins I and II are also present on vesicles, which suggests that these annexins may be involved in vesicular traffic under cell culture conditions.
S Tribolo; S Maroux; D Massey-Harroche
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  78     ISSN:  0829-8211     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-03     Completed Date:  2001-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  495-502     Citation Subset:  IM    
Laboratoire de biologie et de biochimie de la nutrition, URA 1820, Faculté des Sciences de Saint Jérôme, Marseille, France.
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MeSH Terms
Annexins / analysis*,  immunology,  isolation & purification
Blotting, Western
Cell Division
Cell Extracts / analysis
Cells, Cultured
Chelating Agents / pharmacology
Egtazic Acid / pharmacology
Electrophoresis, Gel, Two-Dimensional
Epithelial Cells / chemistry*
Kidney Tubules, Proximal / chemistry*,  cytology*
Microscopy, Fluorescence
Reg. No./Substance:
0/Annexins; 0/Cell Extracts; 0/Chelating Agents; 67-42-5/Egtazic Acid

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