Document Detail


Kidney aminopeptidase A and hypertension, part I: spontaneously hypertensive rats.
MedLine Citation:
PMID:  10024338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue and plasma levels of aminopeptidase A (APA), the principal enzyme that hydrolyzes angiotensin II (Ang II) to angiotensin III, were measured in spontaneously hypertensive rats (SHR) and their normotensive control strain at 3 different ages corresponding to prehypertensive (4 weeks), developing (8 weeks), and established (16 weeks) phases of hypertension. Plasma APA activity was significantly but modestly elevated in SHR at all 3 ages compared with normotensive Wistar-Kyoto rats. Likewise, levels of APA in brain, heart, and adrenal gland were generally, but again only moderately, elevated in SHR at all ages. However, a large increase in APA activity was seen within the kidney in which APA levels were elevated 41%, 51%, and 68% in SHR at 4, 8, and 16 weeks of age, respectively. Kidney APA levels were also significantly increased in immunoblots from 8- and 16-week-old SHR. Glomeruli isolated from 16-week-old SHR had 57% higher APA activity and increased immunoreactivity compared with Wistar-Kyoto rats. To determine whether the increase in kidney APA activity in SHR was related to Ang II levels, SHR were treated for 2 weeks with the angiotensin-converting enzyme inhibitor captopril. Captopril treatment reduced blood pressure to normotensive values and resulted in a 25% reduction in kidney APA activity. These results suggest that APA expression in the kidney may be regulated by activity of the renin-angiotensin system. If so, this would further suggest that upregulation of APA during conditions in which Ang II levels were elevated would have a protective effect against Ang II-mediated cardiovascular diseases, whereas a decrease in APA expression or a failure to upregulate would exacerbate such conditions.
Authors:
D P Healy; L Song
Related Documents :
631188 - Chronic atenolol treatment and hypertension development in spontaneously hypertensive r...
24116178 - Temporal changes of cb1 cannabinoid receptor in the basal ganglia as a possible structu...
23270858 - Adaptive gene expression changes on the healthy side of parkinsonian rats.
966368 - Vascular protein metabolism in the pathogenesis of hypertension.
7276798 - Development of hypothalamic-pituitary-adrenal response to stress in rats made hypothyro...
2774808 - Thyroid function in relation to reproductive efficiency in postpartum buffaloes (bubalu...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  33     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-09     Completed Date:  1999-03-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  740-5     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Mount Sinai School of Medicine of the City University of New York, NY, USA. d_healy@smtplink.mssm.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aminopeptidases / analysis,  metabolism*
Animals
Glutamyl Aminopeptidase
Hypertension / enzymology*,  physiopathology
Kidney / enzymology*,  physiopathology
Male
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Species Specificity
Up-Regulation
Grant Support
ID/Acronym/Agency:
HL42585/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.11.-/Aminopeptidases; EC 3.4.11.7/Glutamyl Aminopeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Angiotensin-converting enzyme is upregulated in the proximal tubules of rats with intense proteinuri...
Next Document:  Kidney aminopeptidase A and hypertension, part II: effects of angiotensin II.