Document Detail

Kidney aminopeptidase A and hypertension, part I: spontaneously hypertensive rats.
MedLine Citation:
PMID:  10024338     Owner:  NLM     Status:  MEDLINE    
Tissue and plasma levels of aminopeptidase A (APA), the principal enzyme that hydrolyzes angiotensin II (Ang II) to angiotensin III, were measured in spontaneously hypertensive rats (SHR) and their normotensive control strain at 3 different ages corresponding to prehypertensive (4 weeks), developing (8 weeks), and established (16 weeks) phases of hypertension. Plasma APA activity was significantly but modestly elevated in SHR at all 3 ages compared with normotensive Wistar-Kyoto rats. Likewise, levels of APA in brain, heart, and adrenal gland were generally, but again only moderately, elevated in SHR at all ages. However, a large increase in APA activity was seen within the kidney in which APA levels were elevated 41%, 51%, and 68% in SHR at 4, 8, and 16 weeks of age, respectively. Kidney APA levels were also significantly increased in immunoblots from 8- and 16-week-old SHR. Glomeruli isolated from 16-week-old SHR had 57% higher APA activity and increased immunoreactivity compared with Wistar-Kyoto rats. To determine whether the increase in kidney APA activity in SHR was related to Ang II levels, SHR were treated for 2 weeks with the angiotensin-converting enzyme inhibitor captopril. Captopril treatment reduced blood pressure to normotensive values and resulted in a 25% reduction in kidney APA activity. These results suggest that APA expression in the kidney may be regulated by activity of the renin-angiotensin system. If so, this would further suggest that upregulation of APA during conditions in which Ang II levels were elevated would have a protective effect against Ang II-mediated cardiovascular diseases, whereas a decrease in APA expression or a failure to upregulate would exacerbate such conditions.
D P Healy; L Song
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Hypertension     Volume:  33     ISSN:  0194-911X     ISO Abbreviation:  Hypertension     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-09     Completed Date:  1999-03-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7906255     Medline TA:  Hypertension     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  740-5     Citation Subset:  IM    
Department of Pharmacology, Mount Sinai School of Medicine of the City University of New York, NY, USA.
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MeSH Terms
Aminopeptidases / analysis,  metabolism*
Glutamyl Aminopeptidase
Hypertension / enzymology*,  physiopathology
Kidney / enzymology*,  physiopathology
Rats, Inbred SHR
Rats, Inbred WKY
Species Specificity
Grant Support
Reg. No./Substance:
EC 3.4.11.-/Aminopeptidases; EC Aminopeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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