Document Detail


Ki67 expression in different epithelial types in columnar lined oesophagus indicates varying levels of expanded and aberrant proliferative patterns.
MedLine Citation:
PMID:  16045773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To evaluate proliferative patterns in metaplastic columnar epithelia of the oesophagus, classified as oxynto-cardiac (n = 43), cardiac (n = 45) intestinal without dysplasia (n = 41), dysplastic intestinal epithelium (n = 25), and adenocarcinoma (n = 15) by Ki67 immunohistochemistry. METHODS AND RESULTS: Abnormal patterns of Ki67 immunoreactivity were classified into (i) expanded proliferation, characterized by increased levels of Ki67 expression in the deep and mid third of the foveolar pit; and (ii) aberrant proliferation, characterized by positive staining in the surface epithelium and superficial third of the foveolar pit. A significant step-wise increase in the frequency of expanded proliferation was seen in oxynto-cardiac, cardiac, intestinal and dysplastic intestinal epithelium indicative of increasing levels of damage. Aberrant proliferation was absent in oxynto-cardiac mucosa, present at a low and similar level in cardiac, intestinal and low-grade dysplastic epithelia and at a significantly increased frequency in high-grade dysplasia. CONCLUSIONS: These findings suggest that oxynto-cardiac mucosa occurs in a low damage environment and intestinal metaplasia in a high damage environment along the length of the columnar lined oesophageal segment. Aberrant proliferative patterns with Ki67 staining are not useful in differentiating reactive epithelia from low-grade dysplasia, but may prove useful in the diagnosis of high-grade dysplasia.
Authors:
M Olvera; K Wickramasinghe; R Brynes; X Bu; Y Ma; P Chandrasoma
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Histopathology     Volume:  47     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-27     Completed Date:  2005-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  132-40     Citation Subset:  IM    
Affiliation:
Department of Pathology, Los Angeles County/University of Southern California Medical Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism,  pathology
Barrett Esophagus / metabolism,  pathology
Cell Proliferation
Diagnosis, Differential
Epithelium / chemistry,  pathology
Esophageal Neoplasms / metabolism,  pathology
Esophagus / chemistry,  pathology*
Humans
Immunohistochemistry
Ki-67 Antigen / analysis*
Chemical
Reg. No./Substance:
0/Ki-67 Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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