Document Detail

Ki-67 labeling in postmitotic cells defines different Ki-67 pathways within the 2c compartment.
MedLine Citation:
PMID:  1935459     Owner:  NLM     Status:  MEDLINE    
Simultaneous quantification of DNA and Ki-67 proliferation-associated antigen was performed using fluorescence image cytometry. In the MCF-7 cell line, the Ki-67 antigen content increases during the cell cycle, and its intranuclear distribution pattern varies. Quantitative evolution of Ki-67 content as a function of nuclear area makes it possible to define several pathways followed by cells going through the 2c compartment. 1) In some cells, the amount of Ki-67 antigen remains constant during G1 (Ki-67 stable pathway), and a characteristic speckled pattern can be observed. 2) In the larger fraction of cells analyzed, there is a postmitotic decrease in the Ki-67 (Ki-67 decrease pathway) content. In this pathway, labeling is located in the nucleoplasm in small nuclei, is located in nucleoli in intermediate-sized nuclei, and is absent from larger nuclei (G0). A progressive increase in Ki-67 content (Ki-67 increase pathway) was observed from intermediate-sized nuclei to S phase nuclei. From these results, we hypothesize that the Ki-67 stable pathway is the G1 phase of newly formed cells going directly to S phase in local optimal conditions of growth and that Ki-67 decrease pathway and Ki-67 increase pathway correspond to cells whose progression to S phase is regulated by extracellular factors.
S du Manoir; P Guillaud; E Camus; D Seigneurin; G Brugal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry     Volume:  12     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1991  
Date Detail:
Created Date:  1991-12-12     Completed Date:  1991-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  455-63     Citation Subset:  IM    
Equipe de Reconnaissance des Fromes et Microscopie Quantitative, TIM3, U.S.R. CNRS B 00690, CERMO Université Joseph Fourier, Grenoble, France.
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MeSH Terms
Breast Neoplasms / chemistry,  metabolism,  pathology*
Cell Cycle
Cell Line
DNA, Neoplasm / analysis
G1 Phase
Image Processing, Computer-Assisted
Immunohistochemistry / methods
Ki-67 Antigen
Nuclear Proteins / metabolism*
S Phase
Reg. No./Substance:
0/DNA, Neoplasm; 0/Ki-67 Antigen; 0/Nuclear Proteins

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