Document Detail


Key role of teichoic acid net charge in Staphylococcus aureus colonization of artificial surfaces.
MedLine Citation:
PMID:  11292767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcus aureus is responsible for a large percentage of infections associated with implanted biomedical devices. The molecular basis of primary adhesion to artificial surfaces is not yet understood. Here, we demonstrate that teichoic acids, highly charged cell wall polymers, play a key role in the first step of biofilm formation. An S. aureus mutant bearing a stronger negative surface charge due to the lack of D-alanine esters in its teichoic acids can no longer colonize polystyrene or glass. The mutation abrogates primary adhesion to plastic while production of the glucosamine-based polymer involved in later steps of biofilm formation is not affected. Our data suggest that repulsive electrostatic forces can lead to reduced staphylococcal biofilm formation, which could have considerable impact on the design of novel implanted materials.
Authors:
M Gross; S E Cramton; F Götz; A Peschel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  69     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-09     Completed Date:  2001-05-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3423-6     Citation Subset:  IM    
Affiliation:
Microbial Genetics, University of Tübingen, D-72076 Tübingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Alanine / physiology*
Bacterial Adhesion
Bacterial Proteins / analysis
Biofilms*
Polysaccharides, Bacterial / biosynthesis
Staphylococcus aureus / physiology*
Static Electricity
Teichoic Acids / chemistry,  pharmacology*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Polysaccharides, Bacterial; 0/Teichoic Acids; 0/polysaccharide intercellular adhesin; 56-41-7/Alanine
Comments/Corrections

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