| Ketone-body utilization by homogenates of adult rat brain. | |
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MedLine Citation:
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PMID: 7121717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The regulation of ketone-body metabolism and the quantitative importance of ketone bodies as lipid precursors in adult rat brain has been studied in vitro. Utilization of ketone bodies and of pyruvate by homogenates of adult rat brain was measured and the distribution of 14C from [3-14C]ketone bodies among the metabolic products was analysed. The rate of ketone-body utilization was maximal in the presence of added Krebs-cycle intermediates and uncouplers of oxidative phosphorylation. The consumption of acetoacetate was faster than that of D-3-hydroxybutyrate, whereas, pyruvate produced twice as much acetyl-CoA as acetoacetate under optimal conditions. Millimolar concentrations of ATP in the presence of uncoupler lowered the consumption of ketone bodies but not of pyruvate. Indirect evidence is presented suggesting that ATP interferes specifically with the mitochondrial uptake of ketone bodies. Interconversion of ketone bodies and the accumulation of acid-soluble intermediates (mainly citrate and glutamate) accounted for the major part of ketone-body utilization, whereas only a small part was oxidized to CO2. Ketone bodies were not incorporated into lipids or protein. We conclude that adult rat-brain homogenates use ketone bodies exclusively for oxidative purposes. |
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Authors:
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M Lopes-Cardozo; W Klein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurochemical research Volume: 7 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1982 Jun |
Date Detail:
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Created Date: 1982-12-02 Completed Date: 1982-12-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 687-703 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dinitrophenol Acetoacetates* Animals Brain / drug effects, metabolism* Carbon Radioisotopes / diagnostic use* Citric Acid Cycle Dinitrophenols / pharmacology Energy Metabolism Keto Acids / metabolism Ketone Bodies / metabolism* Kinetics Magnesium / pharmacology Magnesium Chloride Male Mitochondria / metabolism* Pyruvates / metabolism Pyruvic Acid Rats Rats, Inbred Strains Valinomycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acetoacetates; 0/Carbon Radioisotopes; 0/Dinitrophenols; 0/Keto Acids; 0/Ketone Bodies; 0/Pyruvates; 127-17-3/Pyruvic Acid; 2001-95-8/Valinomycin; 51-28-5/2,4-Dinitrophenol; 541-50-4/acetoacetic acid; 7439-95-4/Magnesium; 7786-30-3/Magnesium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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