Document Detail


Ketoglutarate transport protein KgtP is secreted through the type III secretion system and contributes to virulence in Xanthomonas oryzae pv. oryzae.
MedLine Citation:
PMID:  22685129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phytopathogenic prokaryote Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BB) of rice and utilizes a type III secretion system (T3SS) to deliver T3SS effectors into rice cells. In this report, we show that the ketoglutarate transport protein (KgtP) is secreted in an HpaB-independent manner through the T3SS of X. oryzae pv. oryzae PXO99(A) and localizes to the host cell membrane for α-ketoglutaric acid export. kgtP contained an imperfect PIP box (plant-inducible promoter) in the promoter region and was positively regulated by HrpX and HrpG. A kgtP deletion mutant was impaired in bacterial virulence and growth in planta; furthermore, the mutant showed reduced growth in minimal media containing α-ketoglutaric acid or sodium succinate as the sole carbon source. The reduced virulence and the deficiency in α-ketoglutaric acid utilization by the kgtP mutant were restored to wild-type levels by the presence of kgtP in trans. The expression of OsIDH, which is responsible for the synthesis of α-ketoglutaric acid in rice, was enhanced when KgtP was present in the pathogen. To our knowledge, this is the first report demonstrating that KgtP, which is regulated by HrpG and HrpX and secreted by the T3SS in Xanthomonas oryzae pv. oryzae, transports α-ketoglutaric acid when the pathogen infects rice.
Authors:
Wei Guo; Lu-Lu Cai; Hua-Song Zou; Wen-Xiu Ma; Xi-Ling Liu; Li-Fang Zou; Yu-Rong Li; Xiao-Bin Chen; Gong-You Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-08
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-11-28     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5672-81     Citation Subset:  IM    
Affiliation:
Laboratory for Monitoring and Management for Plant Pests and Diseases (Ministry of Education of China), College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Secretion Systems*
Carbon / metabolism
Culture Media / chemistry
Dicarboxylic Acid Transporters / genetics,  secretion*
Gene Deletion
Genetic Complementation Test
Ketoglutaric Acids / metabolism
Oryza sativa / microbiology*
Plant Diseases / microbiology*
Succinic Acid / metabolism
Virulence
Virulence Factors / genetics,  metabolism*
Xanthomonas / growth & development,  metabolism,  pathogenicity*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Dicarboxylic Acid Transporters; 0/Ketoglutaric Acids; 0/Virulence Factors; 110-15-6/Succinic Acid; 7440-44-0/Carbon
Comments/Corrections

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