| Keto fatty acids not containing doubly allylic methylenes are lipoxygenase substrates. | |
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MedLine Citation:
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PMID: 1931955 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The soybean lipoxygenase I oxygenates the unusual substrate 12-keto-(9Z)-octadecenoic acid methyl ester as indicated by oxygen uptake and spectral changes of the incubation mixture. The main oxygenation products have been isolated by HPLC and identified as 9,12-diketo-(10E)-octadecenoic acid methyl ester and 12-keto-(10E)-dodecenoic acid methyl ester by UV and IR spectroscopy, cochromatography with an authentic standard, gas chromatography/mass spectroscopy, and 1H NMR. In the formation of both compounds the oxygenase and hydroperoxidase activities of the enzyme appear to be involved. These data and the earlier results on the oxygenation of furanoic fatty acids (Boyer et al., 1979) indicate that the lipoxygenase reaction is not restricted to substrates containing a 1,4-pentadiene structure. |
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Authors:
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H Kühn; L Eggert; O A Zabolotsky; G I Myagkova; T Schewe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry Volume: 30 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-11-25 Completed Date: 1991-11-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 10269-73 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry, Medical School (Charité), Humboldt University, Berlin, FRG. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fatty Acids
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chemistry* Lipoxygenase / chemistry* Oxidation-Reduction Soybeans / enzymology Structure-Activity Relationship Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; EC 1.13.11.12/Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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