Document Detail

Keratocyte density: comparison of two confocal microscopes.
MedLine Citation:
PMID:  15665669     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The ConfoScan 3 clinical confocal microscope provides sharper images of cells in the stroma than the Tandem Scanning confocal microscope does. In this study, we compared volumetric densities of stromal cells determined from images recorded by these two instruments. METHODS: Fifty corneas of 25 normal subjects were examined by confocal microscopy, first by using a Tandem Scanning confocal microscope and then by using a ConfoScan 3 confocal microscope. Bright objects, assumed to represent keratocytes, were counted in a known area of two frames selected from the mid-stroma. The effective depth of the sample volume represented by each frame was estimated from the number of consecutive frames and the corresponding distance that selected cells were visible and countable during a scan. Density was the number of visible cells in the sample area divided by the effective sample volume. RESULTS: The effective focal depth of the Tandem Scanning microscope was 11.9 +/- 2.6 microm (mean +/- SD), and was 25.9 +/- 7.1 microm for the ConfoScan 3. Mean cell density at mid stroma was 23,013 +/- 4,420 cells/mm(3) with the Tandem Scanning microscope and 23,996 +/- 2,898 cells/mm(3) with the ConfoScan 3. This difference was not significant (P = 0.15). CONCLUSIONS: In normal corneas, the ConfoScan 3 and the Tandem Scanning confocal microscopes indicate stromal cell densities that are not significantly different from each other. Estimates of cell density from both instruments require an accurate estimate of the effective depth of the sample volume; this depth is approximately 2.2 times greater with the ConfoScan 3. This difference must be considered when comparing results from studies that use one instrument with results from studies that use the other.
Jay W McLaren; Cherie B Nau; Anna S Kitzmann; William M Bourne
Related Documents :
7420049 - Control of active proton transport in turtle urinary bladder by cell ph.
495119 - The role of blood coagulability and axial streaming of erythrocytes in determining f ce...
9634209 - Intracellular ph and chemoresponse to nh4+ in paramecium.
6292509 - Poliovirus-induced alterations in hela cell membrane functions.
16988859 - Lack of threshold for anisotonic cell volume regulation.
12117229 - Changes of element ratios of cultured cells from dune reed under adverse environmental ...
23053899 - Sulf1 inhibits proliferation and invasion of esophageal squamous cell carcinoma cells b...
10523849 - Relative level of expression of bax and bcl-xl determines the cellular fate of apoptosi...
8496779 - Cell cycle-specific growth inhibitory effect on human gingival fibroblasts of a toxin i...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Eye & contact lens     Volume:  31     ISSN:  1542-2321     ISO Abbreviation:  Eye Contact Lens     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-24     Completed Date:  2005-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101160941     Medline TA:  Eye Contact Lens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-33     Citation Subset:  IM    
Department of Ophthalmology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Count
Corneal Stroma / cytology*
Microscopy, Confocal / instrumentation*
Middle Aged
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Corneal oxygen deficiency.
Next Document:  Effect of preservative-free artificial tears on the antimicrobial activity of human beta-defensin-2 ...