| Keratinocytes modify fibroblast metabolism in hereditary gingival fibromatosis. | |
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MedLine Citation:
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PMID: 18589399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Hereditary gingival fibromatosis (HGF) is a rare benign disorder characterized by progressive fibrous overgrowth of the gingiva. The proliferation and expression of growth factors of HGF keratinocytes are abnormal. However, the exact role of keratinocytes in HGF pathogenesis is still unknown. The present study aimed to clarify the interactions between HGF keratinocytes and underlying fibroblasts in the pathogenesis of HGF. DESIGN: Gingival tissues, fibroblasts and keratinocytes from three Chinese HGF patients and three healthy subjects were collected. Histological analyses were performed by histochemical and immunohistochemical staining (Ki-67). Gingival fibroblasts were cocultured with gingival keratinocytes in an in vitro coculture system. The mRNA levels of type I collagen, MMP-1, MMP-3, and TIMP-1 were analysed in the cocultured gingival fibroblasts by reverse-transcription polymerase chain reaction (RT-PCR). The production of type I collagen and TIMP-1 was examined by ELISA. RESULTS: The number of Ki-67-positive keratinocytes in tissue sections from patients was higher than in those from controls. HGF fibroblasts cocultured with HGF keratinocytes showed an increased expression of type I collagen and TIMP-1. Transmission electron microscopy showed increased rough endoplasmic reticulum and ribosomes in cocultured HGF fibroblasts. CONCLUSIONS: These results suggest that HGF keratinocytes have an important role in HGF pathogenesis by inducing extracellular matrix (ECM) accumulation by fibroblasts. |
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Authors:
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Liuyan Meng; Xiaoqian Ye; Mingwen Fan; Xuepeng Xiong; Johannes W Von den Hoff; Zhuan Bian |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-27 |
Journal Detail:
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Title: Archives of oral biology Volume: 53 ISSN: 1879-1506 ISO Abbreviation: Arch. Oral Biol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-09-16 Completed Date: 2009-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: England |
Other Details:
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Languages: eng Pagination: 1050-7 Citation Subset: D; IM |
Affiliation:
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Key Laboratory for Oral Biomedical Engineering Ministry of Education, School and Hospital of Stomatology, Wuhan University, Luoyu Road 237, 430079 Wuhan, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Cell Culture Techniques Coculture Techniques Collagen Type I / biosynthesis, genetics Female Fibroblasts / metabolism*, ultrastructure Fibromatosis, Gingival / genetics, metabolism, pathology* Gene Expression Gingiva / metabolism, ultrastructure Humans Keratinocytes / physiology* Male Matrix Metalloproteinase 1 / biosynthesis, genetics Matrix Metalloproteinase 3 / biosynthesis, genetics Microscopy, Electron RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction / methods Tissue Inhibitor of Metalloproteinase-1 / biosynthesis, genetics Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; EC 3.4.24.17/Matrix Metalloproteinase 3; EC 3.4.24.7/Matrix Metalloproteinase 1 |
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