Document Detail


Keratinocytes efficiently process endogenous antigens for cytotoxic T-cell mediated lysis.
MedLine Citation:
PMID:  19601980     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The generation of a robust CD8(+) cytotoxic T lymphocyte (CTL) response is a key feature of many immunotherapeutic strategies against epithelial tumors and virally infected epithelial tissue. However, surprisingly few studies have addressed whether primary epithelial cells, expressing defined endogenous antigens, are good targets for CTL-mediated lysis. Here, we show that primary keratinocytes (KCs), expressing endogenous ovalbumin (OVA) as a transgene, present measurable H-2K(b)/SIINFEKL complexes at the cell surface and are killed by OVA-specific CTL. Target cell lysis was comparable with a more traditional CTL target cell, EL4, and was enhanced by KC pretreatment with interferon-gamma. These results suggest that primary KCs will be susceptible to CTL-mediated apoptosis when endogenous KC antigens are targeted during immunotherapy.
Authors:
Fang Zhou; Ian H Frazer; Graham R Leggatt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  18     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-05-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  1053-9     Citation Subset:  IM    
Affiliation:
The University of Queensland Diamantina Institute for Cancer, Immunology and Metabolic Medicine, Princess Alexandra Hospital, Brisbane, Qld, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antigen Presentation / immunology*
Cell Line, Tumor
Cells, Cultured
Cytotoxicity Tests, Immunologic
Cytotoxicity, Immunologic / immunology*
H-2 Antigens / metabolism
Interferon-gamma / pharmacology
Keratinocytes / drug effects,  immunology*,  metabolism
Keratins / genetics
Mice
Mice, Inbred C57BL
Mice, Transgenic
Ovalbumin / genetics,  immunology,  metabolism
Peptide Fragments / genetics,  immunology,  metabolism
Promoter Regions, Genetic / genetics
T-Lymphocytes, Cytotoxic / immunology*
Vaccination
Chemical
Reg. No./Substance:
0/H-2 Antigens; 0/H-2Kb protein, mouse; 0/OVA-8; 0/Peptide Fragments; 68238-35-7/Keratins; 82115-62-6/Interferon-gamma; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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