| Keratinocytes drive melanoma invasion in a reconstructed skin model. | |
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MedLine Citation:
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PMID: 20700063 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Melanoma progression is a multistep progression from a common melanocytic nevus through the radial growth phase, the invasive vertical growth phase finally leading to metastatic spread into distant organs. Migration and invasion of tumor cells requires secretion of proteases to facilitate remodeling of the extracellular matrix including basement membranes. Here we used a reconstructed skin model to investigate melanoma growth and invasion in vitro. Using this model we show that the dermoepidermal basement membrane prevents the invasion of metastatic melanoma BLM and MV3 cells in the absence of a stratified epidermis. In the reconstructed skin model, matrix metalloproteinase-9, a protease activated early in melanoma development, is secreted by the keratinocytes and subsequently activated by an unknown soluble factor secreted by the melanoma cells. The dynamic interplay between keratinocytes and melanoma cells is further shown by an altered growth pattern of melanoma cells and the finding that a reconstructed epidermis induces invasion. Overall, our findings show that the invasive behavior of melanoma cells is determined by the melanoma cells themselves, but that the interplay between surrounding keratinocytes and the melanoma cells plays an important role in melanoma invasion. |
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Authors:
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Jeroen W J Van Kilsdonk; Mieke Bergers; Léon C L T Van Kempen; Joost Schalkwijk; Guido W M Swart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Melanoma research Volume: 20 ISSN: 1473-5636 ISO Abbreviation: Melanoma Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-09 Completed Date: 2011-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9109623 Medline TA: Melanoma Res Country: England |
Other Details:
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Languages: eng Pagination: 372-80 Citation Subset: IM |
Affiliation:
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Department of Biomolecular Chemistry, Faculty of Science, Radboud University, Nijmegen, The Netherlands. J.vankilsdonk@derma.umcn.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Communication
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physiology Cell Proliferation Cells, Cultured Humans Keratinocytes / pathology, physiology* Melanoma / pathology* Models, Biological Neoplasm Invasiveness Neoplasm Metastasis Organ Culture Techniques / methods Skin* / pathology Skin Neoplasms / pathology* Skin Physiological Processes Tissue Engineering / methods Tissue Scaffolds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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