Document Detail


Keratinocyte growth factor-2 accelerates wound healing in incisional wounds.
MedLine Citation:
PMID:  9927546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Keratinocyte growth factor-2 (KGF-2) also described as fibroblast growth factor-10 (FGF-10) is a newly identified member of the fibroblast growth factor family. KGF-2 is 96% identical to the recently identified rat FGF-10 and specifically stimulates growth of normal human epidermal keratinocytes. The present study was undertaken to examine the effects of topically applied KGF-2 in an incisional wound healing model. KGF-2 treatment resulted in an improvement in incisional wound healing as characterized by an increase in breaking strength, collagen content, and epidermal thickness. METHODS: KGF-2 was topically applied to linear incisions made in the dorsal skin of Sprague-Dawley rats. Biomechanical testing was done using an Instron tensiometer for breaking and tensile strength determinations. Wound collagen content was determined using the Sircol collagen assay. Epidermal thickness measurements were conducted using Masson's trichrome-stained sections of the wound. RESULTS: A single topical application of KGF-2 at the time of wounding resulted in an increase in wound breaking and tensile strength at Day 5 after wounding. Breaking strength of KGF-2-treated wounds was significantly higher compared with the buffer control (1 microgram, 222.1 +/- 13.5 g, P = 0.0007; 4 microgram, 248.7 +/- 15.4 g, P = 0.0001; 10 microgram, 247.2 +/- 21.9 g, P = 0.001; buffer, 141.0 +/- 9.7 g). Epidermal thickness and wound collagen content were significantly increased following treatment with KGF-2. CONCLUSIONS: Based on our findings, KGF-2 is a potent stimulator of wound healing as demonstrated by increased mechanical strength accompanied by an increase in wound collagen content. KGF-2 could be an important cellular mediator responsible for the initiation and acceleration of wound healing and may enhance the healing of surgical wounds.
Authors:
P A Jimenez; M A Rampy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  81     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-02-25     Completed Date:  1999-02-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  238-42     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Human Genome Sciences, Inc., Rockville, Maryland, 20850, USA. jimenez@hgsi.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Fibroblast Growth Factor 10
Fibroblast Growth Factor 7
Fibroblast Growth Factors*
Growth Substances / pharmacology*
Humans
Keratinocytes / cytology
Male
Rats
Rats, Sprague-Dawley
Recombinant Proteins / pharmacology
Skin / drug effects,  injuries*
Skin Physiological Phenomena / drug effects
Tensile Strength
Wound Healing / drug effects*,  physiology
Chemical
Reg. No./Substance:
0/FGF10 protein, human; 0/FGF7 protein, human; 0/Fgf10 protein, rat; 0/Fgf7 protein, rat; 0/Fibroblast Growth Factor 10; 0/Growth Substances; 0/Recombinant Proteins; 126469-10-1/Fibroblast Growth Factor 7; 62031-54-3/Fibroblast Growth Factors

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