Document Detail


Keratin immunohistochemistry in normal human liver. Cytokeratin pattern of hepatocytes, bile ducts and acinar gradient.
MedLine Citation:
PMID:  2446418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A panel of 2 polyclonal and 7 monoclonal antibodies directed against cytokeratins was tested on cryostat and paraffin sections of 14 normal human liver biopsies using an immunoperoxidase procedure. The staining characteristics of hepatocytes and bile ducts are reported. On cryostat sections, monoclonal antibodies directed against individual cytokeratins no. 8 and no. 18 stained both bile ducts and hepatocytes, whereas monoclonals anti-cytokeratin no. 7 and no. 19 exclusively stained bile ducts. The potential use of these 4 monoclonal antibodies in liver histopathology is briefly discussed. Monoclonal antibody anti-type II cytokeratins and the polyclonal rabbit anti-human keratin stained only bile ducts on both cryostat and paraffin sections. Using monoclonal antibody CAM 5.2 on paraffin sections, both bile ducts and parenchyma were positive. An acinar gradient was apparent in that zone 1 hepatocytes were more intensely stained. Moreover, a rim of hepatocytes around terminal hepatic venules and adjacent to subhepatic veins showed more intense staining. The same gradient could be seen in some paraffin sections stained with the monoclonals anti-cytokeratin no. 18 and KL1, and the rabbit polyclonal anti-keratin "wide spectrum screening". The gradient is interpreted as reflecting quantitative differences in keratin content between hepatocytes. Polyclonal rabbit anti-human keratin is proposed as the most reliable antibody for identification of bile ducts in paraffin sections. The usefulness of reliable bile duct staining in several pathological conditions is emphasized.
Authors:
P van Eyken; R Sciot; B van Damme; C de Wolf-Peeters; V J Desmet
Related Documents :
14234808 - Use of a fluorescein-labeled sonically disrupted bacterial antigen to demonstrate antib...
18827478 - Effects of fixative and embedding medium on morphology and immunostaining of the cochlea.
2079108 - Enhanced immunocytochemical staining sensitivity in formalin-fixed and paraffin-embedde...
2820908 - Simultaneous demonstration of two antigens in ultrathin cryosections by a novel applica...
15377288 - Decreased expression of b7 costimulatory molecules and major histocompatibility complex...
262448 - Regulation of ige antibody production by serum molecules. vi: preliminary biochemical a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virchows Archiv. A, Pathological anatomy and histopathology     Volume:  412     ISSN:  0174-7398     ISO Abbreviation:  Virchows Arch A Pathol Anat Histopathol     Publication Date:  1987  
Date Detail:
Created Date:  1988-01-13     Completed Date:  1988-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302198     Medline TA:  Virchows Arch A Pathol Anat Histopathol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  63-72     Citation Subset:  IM    
Affiliation:
Department of Pathology, Universitair Ziekenhuis St. Rafaël, Leuven, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / analysis
Bile Ducts, Intrahepatic / cytology,  metabolism*
Biopsy
Humans
Immunohistochemistry
Keratins / immunology,  metabolism*
Liver / cytology,  metabolism*
Reference Values
Staining and Labeling / methods
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Analysis of pancreatic islet cells and hormone content in the spontaneously diabetic KKAy mouse by m...
Next Document:  Chronic sialadenitis. An immunocytochemical study in humans.