| Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming. | |
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MedLine Citation:
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PMID: 22522173 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transcription-factor-directed reprogramming from somatic cells to induced pluripotent stem cells (iPSCs) is by nature an epigenetic process of cell fate change. Previous studies have demonstrated that this inefficient process can be facilitated by the inclusion of additional factors. To gain insight into the reprogramming mechanism, we aimed to identify epigenetic enzymes capable of promoting iPSC generation. Here we show that Kdm2b, a histone H3 Lys 36 dimethyl (H3K36me2)-specific demethylase, has the capacity to promote iPSC generation. This capacity depends on its demethylase and DNA-binding activities, but is largely independent of its role in antagonizing senescence. Kdm2b functions at the beginning of the reprogramming process and enhances activation of early responsive genes in reprogramming. Kdm2b contributes to gene activation by binding to and demethylating the gene promoters. Our studies not only identify an important epigenetic factor for iPSC generation, but also reveal the molecular mechanism underlying how Kdm2b contributes to reprogramming. |
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Authors:
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Gaoyang Liang; Jin He; Yi Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-04-22 |
Journal Detail:
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Title: Nature cell biology Volume: 14 ISSN: 1476-4679 ISO Abbreviation: Nat. Cell Biol. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-04 Completed Date: 2012-06-29 Revised Date: 2013-02-21 |
Medline Journal Info:
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Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 457-66 Citation Subset: IM |
Affiliation:
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Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Proliferation Epigenesis, Genetic F-Box Proteins / physiology* Gene Expression Regulation* Jumonji Domain-Containing Histone Demethylases / physiology* Mice Pluripotent Stem Cells / cytology* Polymerase Chain Reaction Promoter Regions, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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U01 DK089565/DK/NIDDK NIH HHS; U01DK089565/DK/NIDDK NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/F-Box Proteins; EC 1.14.11.-/Jumonji Domain-Containing Histone Demethylases; EC 1.14.11.27/Kdm2b protein, mouse |
| Comments/Corrections | |
Erratum In:
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Nat Cell Biol. 2012 Jun;14(6):649 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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