Document Detail

Kazakh therapy on differential protein expression of Achilles tendon healing in a 7-day postoperative rabbit model.
MedLine Citation:
PMID:  22462247     Owner:  NLM     Status:  In-Process    
OBJECTIVE: To compare the effect of cast immobilization with that of early Kiymil arkili emdew (Kazakh exercise therapy) on the post-operative healing of Achilles tendon rupture in rabbits, and to observe the influence of early Kiymil arkili emdew on the differentially expressed proteins in the healing tendon.
METHODS: Forty-five New Zealand white rabbits were randomly divided into three groups (Arm A: control group; Arm B: postoperative immobilization group; and Arm C: postoperative early Kiymil arkili emdew group). After tenotomy, the rabbits of the two experimental groups received microsurgery to repair the ruptured tendons, and then received either cast immobilization or early Kiymil arkili emdew treatment. Achilles tendon tissue samples were collected 7 days after the surgery, and two-dimensional gel electrophoresis and MALDI-TOF-MS technique were used to analyze differentially expressed proteins in the tendon tissue of the three Arms.
RESULTS: A total of 462.67 +/- 11.59, 532.33 +/- 27.79, and 515.33 +/- 6.56 protein spots were detected by the two-dimensional polyacrylamide gels in the Achilles tendon samples of the rabbits in Arms A, B, and C, respectively. Nineteen differentially expressed protein spots were randomly selected from Arm C. Among them, 7 were unique, and 15 had five times higher abundance than those in Arm B. These included annexin A2, gelsolin isoforms and alpha-1 Type III collagen. It was confirmed by western blot that gelsolin isoform b, annexin A2, etc. had specific and incremental expression in Arm C.
CONCLUSIONS: The self-protective instincts of humans were overlooked in the classical postoperative treatment for Achilles tendon rupture with cast immobilization. Kiymil arkili emdew induced the specific and incremental expression of proteins in the repaired Achilles tendon in the early healing stage in a rabbit model, compared with those treated with postoperative cast immobilization. These differentially expressed proteins may contribute to the healing of the Achilles tendon via a mechanobiological mechanism caused by the application of Kiymil arkili emdew.
Shawutali Nuerai; Jialili Ainuer; Jielile Jiasharete; Redati Darebai; Aldyarhan Kayrat; Bin Tang; Zheyiken Jiangannur; Jingping Bai; Bolat Makabel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan / sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine     Volume:  31     ISSN:  0255-2922     ISO Abbreviation:  J Tradit Chin Med     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-04-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211546     Medline TA:  J Tradit Chin Med     Country:  China    
Other Details:
Languages:  eng     Pagination:  367-75     Citation Subset:  IM    
Department of Microsurgical Repair and Reconstruction, the First Teaching Hospital of Xinjiang Medical University & Sports Medicine Research Center, Research Institute of Orthopedics of Xinjiang Uyghur Autonomous Region, Urumqi 830054, China.
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