Document Detail


Karyotyping of individual cells with flow cytometry.
MedLine Citation:
PMID:  8900470     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As the study of metaphase chromosomes with flow cytometry presupposes mixing all chromosomes from many cells before analysis, important information is lost. To overcome this limitation we have developed a novel cell-oriented flow cytometric method for chromosome analysis. A flow cytometer supplied with a special device for disruption of metaphase chromosomes is the heart of the method. Cells with stained chromosomes are pressed into the disruption device and then converted in batches of chromosomes. Chromosome fluorescence and time intervals between fluorescence pulses are registered. There are large time intervals between neighboring batches because a sparse cell suspension is used and, in turn, there are small time intervals inside batches. Taking account of time intervals, it is possible to identify individual cell flow karyotypes. This highly productive method for individual cell analysis (100-200 cells/min) ensures detecting the changes in cell flow karyotypes, i.e., under- and overrepresentation of chromosomes, aberrations, and amplification of DNA.
Authors:
S I Stepanov; V N Konyshev; L V Kotlovanova; A P Roganov
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cytometry     Volume:  23     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-12-23     Completed Date:  1996-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  279-83     Citation Subset:  IM    
Affiliation:
Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute, Leningrad, Russia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cells
Chromosomes
Cricetinae
Flow Cytometry / methods*
Karyotyping*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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