Document Detail

Karyometric and cytophotometric studies on nucleic acids in the culture of human lymphocytes with phytohemagglutinin.
MedLine Citation:
PMID:  824810     Owner:  NLM     Status:  MEDLINE    
Lymphocyte transformation with phytohemagglutinin (PHA) was analyzed by karyometry and cytophotometry of single cells. The former showed the distribution of various-sized transforming cells, and the latter the increase of DNA and RNA. Two different types of karyograms were obtained; one with three peaks corresponding to 2c, 4c, and 8c, and the other with only a 2c peak. Although small in number (8%), karyometry showed that DNA synthesis took place in the extremely early stage of transformation, 1 h after stimulation with PHA, which was proved by cytophotometry and ultrastructural findings. An increasing curve of DNA and RNA revealed that a lymphocyte continued to enlarge from 2c to 4c and divide again through the normal cell cycle in a suitable condition. The largest blast cell, a polyploid cell, may be induced by the lack of a sufficient amount of RNA and protein in need of cell division, and may be a degenerative cell in the strict sense. Among the large cells with blastlike appearance, there were a considerable number of so-called degenerative cells with a low amount of DNA. The ratio of RNA to DNA increased steeply in the actively DNA synthesizing S phase, which supports the morphologic findings of blast cell, the appearance of basophilic cytoplasm, and active-looking nucleoli.
A Takahashi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virchows Archiv. B: Cell pathology     Volume:  21     ISSN:  -     ISO Abbreviation:  Virchows Arch B Cell Pathol     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1976-12-23     Completed Date:  1976-12-23     Revised Date:  2014-07-21    
Medline Journal Info:
Nlm Unique ID:  0437105     Medline TA:  Virchows Arch B Cell Pathol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  299-311     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cell Nucleolus
Lectins / pharmacology*
Lymphocyte Activation
Lymphocytes / analysis,  drug effects*
Nucleic Acids / analysis*
Time Factors
Reg. No./Substance:
0/Lectins; 0/Nucleic Acids

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