Document Detail

Kainic acid, AMPA, and dihydrokainic acid effect on uptake and efflux of D-[3H] aspartic acid in cerebellar slices.
MedLine Citation:
PMID:  8953569     Owner:  NLM     Status:  MEDLINE    
In this study we show that the glutamate ionotropic agonist kainate (KA) stimulates the efflux of preloaded D-[3H]aspartate (D-[3H]Asp) and inhibits the uptake of this amino acid in cerebellar slices. The effect of this agonist on the efflux of D-[3H]Asp is sensitive to (i) 6-nitro-7-sulphamoylbenzo(f)quinoxaline-2-3-dione (NBQX), indicating the involvement of KA/(RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, and is (ii) partially tetrodotoxin (TTX)-sensitive, indicating that pre-(TTX-insensitive) and post-synaptic (TTX-sensitive) KA/AMPA receptors are involved. In contrast, the effect on uptake is NBQX- and TTX-insensitive indicating a direct interaction with glutamate transporters. AMPA inhibited D-[3H]Asp uptake and had no effect on D-[3H]Asp efflux. In the same system, the uptake but not the efflux of D-[3H]Asp was affected by dihydrokainate (DHK). The DHK-induced uptake inhibition occurred in the presence of TTX. NBQX inhibited DHK-induced effect at 5 mM but not at 1 mM DHK concentrations.
M Bouazzaoui; C Kannengieser; O Procksch; G Gombos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemical research     Volume:  21     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-03-25     Completed Date:  1997-03-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1527-33     Citation Subset:  IM    
Laboratoire de Neurobiologie Cellulaire, UPR 9009 CNRS, Centre de Neurochimie, Strasbourg, France.
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MeSH Terms
Aspartic Acid / metabolism*
Cerebellum / drug effects*,  metabolism*
Excitatory Amino Acid Agonists / pharmacology
Excitatory Amino Acid Antagonists / pharmacology
Kainic Acid / analogs & derivatives*,  pharmacology*
Quinoxalines / pharmacology
Rats, Wistar
Tetrodotoxin / pharmacology
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology*
Reg. No./Substance:
0/Excitatory Amino Acid Agonists; 0/Excitatory Amino Acid Antagonists; 0/Quinoxalines; 10028-17-8/Tritium; 118876-58-7/2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline; 4368-28-9/Tetrodotoxin; 487-79-6/Kainic Acid; 52497-36-6/dihydrokainic acid; 56-84-8/Aspartic Acid; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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