Document Detail


Kaempferol-7-O-beta-D-glucoside (KG) isolated from Smilax china L. rhizome induces G2/M phase arrest and apoptosis on HeLa cells in a p53-independent manner.
MedLine Citation:
PMID:  18343026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kaempferol-7-O-beta-D-glucoside (KG), a flavonoid glycoside, isolated from Smilax china L. rhizome, displayed marked anticancer activity on a panel of established cancer cells, of which, HeLa human cervix carcinoma cells were the most sensitive. Meanwhile, the cytotoxic effects of KG on normal human cells (HEK293 embryonic kidney cells and L-02 embryonic liver cells) were much smaller than on cancer cells. This work studied the molecular mechanisms underlying KG induced growth inhibition in HeLa cells. The results showed that KG induced G2/M phase growth arrest correlated with Cyclin B1 and Cdk1 decrease in a p53-independent manner, and also caused an increase in apoptosis, which was confirmed by characteristic morphological changes, evident DNA fragmentation, increased apoptotic sub-G1 population. Furthermore, inhibition of NF-kappaB nuclear translocation, up-regulation of Bax and down-regulation of Bcl-2, were observed in HeLa cells treated with KG, which indicated that the mitochondrial pathway was involved in the apoptosis signal pathway. In summary, KG displayed a significant anti-tumor effect through cell cycle arrest and apoptotic induction in HeLa cells, which suggested that KG might have therapeutic potential against cervix carcinoma.
Authors:
Wen Xu; Jianwen Liu; Changlong Li; He-Zhen Wu; Yan-Wen Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-14
Journal Detail:
Title:  Cancer letters     Volume:  264     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-06     Completed Date:  2008-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  229-40     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Bioreactor Engineering & School of pharmacy, East China University of Science and Technology, #268, 130 Meilong Road, Shanghai 200237, PR China.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Blotting, Western
Cell Cycle / drug effects*
Cell Proliferation / drug effects
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / pharmacology*
Flow Cytometry
Glycosides / pharmacology*
Hela Cells
Humans
Kaempferols / pharmacology*
NF-kappa B / drug effects,  metabolism
Phytotherapy*
Proto-Oncogene Proteins c-bcl-2 / drug effects,  metabolism
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Rhizome / chemistry
Smilax / chemistry*
Tumor Suppressor Protein p53 / drug effects,  metabolism
bcl-2-Associated X Protein / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Drugs, Chinese Herbal; 0/Glycosides; 0/Kaempferols; 0/NF-kappa B; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; 0/bcl-2-Associated X Protein; 0/kaempferol-3-O-(6-trans-caffeoyl)-beta-D-glucopyranosyl-(1- 2)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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