Document Detail

KITLG is a novel target of miR-34c that is associated with the inhibition of growth and invasion in colorectal cancer cells.
MedLine Citation:
PMID:  25213795     Owner:  NLM     Status:  In-Process    
MiR-34c is considered a potent tumour suppressor because of its negative regulation of multiple target mRNAs that are critically associated with tumorigenesis and metastasis. In the present study, we demonstrated a novel target of miR-34c, KITLG, which has been implicated in colorectal cancer (CRC). First, we found a significant negative relationship between miR-34c and KITLG mRNA expression levels in CRC cell lines, including HT-29, HCT-116, SW480 and SW620 CRC cell lines. In silico analysis predicted putative binding sites for miR-34c in the 3' untranslated region (3'UTR) of KITLG mRNA. A dual-luciferase reporter assay further confirmed that KITLG is a direct target of miR-34c. Then, the cell lines were infected with lentiviruses expressing miR-34c or a miR-34c specific inhibitor. Restoration of miR-34c dramatically reduced the expression of KITLG mRNA and protein, while silencing of endogenous miR-34c increased the expression of KITLG protein. The miR-34c-mediated down-regulation of KITLG was associated with the suppression on proliferation, cellular transformation, migration and invasion of CRC cells, as well as the promotion on apoptosis. Knockdown of KITLG by its specific siRNA confirmed a critical role of KITLG down-regulation for the tumour-suppressive effects of miR-34c in CRC cells. In conclusion, our results demonstrated that miR-34c might interfere with KITLG-related CRC and could be a novel molecular target for CRC patients.
Shu Yang; Wen-shuai Li; Fang Dong; Hai-mei Sun; Bo Wu; Jun Tan; Wan-jing Zou; De-shan Zhou
Related Documents :
15961395 - C-jun/activator protein-1 mediates interleukin-1beta-induced dedifferentiation but not ...
8344255 - Mhc class i gene expression is negatively regulated by the proto-oncogene, c-jun.
19912895 - Regulation of proenkephalin expression in c6 rat glioma cells.
16732985 - Uvb-irradiated human keratinocytes and interleukin-1alpha indirectly increase map kinas...
3044365 - Insulin-mediated post-transcriptional regulation of hepatic malic enzyme and albumin mr...
11675495 - A conditional tissue-specific transgene expression system using inducible gal4.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2014-09-12
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  18     ISSN:  1582-4934     ISO Abbreviation:  J. Cell. Mol. Med.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-09     Completed Date:  -     Revised Date:  2014-12-04    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  2092-102     Citation Subset:  IM    
Copyright Information:
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Mineral phases and mobility of trace metals in white aluminum precipitates found in acid mine draina...
Next Document:  pH Stability Study of Lectin from Black Turtle Bean (Phaseolus Vulgaris) as Influenced by Guanidiniu...