Document Detail

KCNA1 and TRPC6 ion channels and NHE1 exchanger operate the biological outcome of HGF/scatter factor in renal tubular cells.
MedLine Citation:
PMID:  18365869     Owner:  NLM     Status:  MEDLINE    
Hepatocyte growth factor (HGF) is a glycoprotein that induces in vitro epithelial tubular cell growth, motility, scattering and branching morphogenesis. The cell machineries that account for HGF biological effects are still unclear. In previous study, we found that HGF upregulated in epithelial tubular cell line (HK2) 3 genes: potassium channel KCNA1, calcium channel (transient receptor potential channel, subfamily C, member 6, TRPC6) and Na(+)/H(+) exchanger-1 (NHE1). In this study, we validated these results with reverse transcription PCR and WB analysis. To investigate whether KCNA1, TRPC6, NHE1 mediate the changes induced by HGF in HK2, we studied the effects of their inhibitors: 4-aminopyridine, charybdotoxin, dendrotoxin K inhibitors of KCNA1, lanthanum, N-(p-amylcinnamoyl) anthranilic acid inhibitors of TRPC6, 5-(N-ethyl-N-isopropyl)amiloride, cariporide inhibitors of NHE1. The inhibitors prevented HGF-induced growth, migration, cytoskeletal reorganization and tubulogenesis in HK2. These results indicate that KCNA1, TRPC6 and NHE1 are cell machineries that are exploited by HGF to effect its biological outcome in renal tubular cells.
Teresa Rampino; Marilena Gregorini; Cristina Guidetti; Massimo Broggini; Sergio Marchini; Riccardo Bonomi; Milena Maggio; Elisabetta Roscini; Grazia Soccio; Renza Tiboldo; Antonio Dal Canton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Growth factors (Chur, Switzerland)     Volume:  25     ISSN:  0897-7194     ISO Abbreviation:  Growth Factors     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2008-03-27     Completed Date:  2008-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9000468     Medline TA:  Growth Factors     Country:  England    
Other Details:
Languages:  eng     Pagination:  382-91     Citation Subset:  IM    
Unit of Nephrology, Dialysis and Transplantation, Fondazione, IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy.
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MeSH Terms
Calcium Channel Blockers / pharmacology
Cation Transport Proteins / physiology*
Cell Line
Cell Movement
Cell Proliferation / drug effects
Cytoskeleton / metabolism
Hepatocyte Growth Factor / pharmacology,  physiology*
Ion Channel Gating / drug effects,  physiology*
Kidney Tubules / cytology
Kv1.1 Potassium Channel / physiology*
Potassium Channel Blockers / pharmacology
Signal Transduction
Sodium Channel Blockers / pharmacology
Sodium-Hydrogen Antiporter / physiology*
TRPC Cation Channels / physiology*
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Cation Transport Proteins; 0/HGF protein, human; 0/KCNA1 protein, human; 0/Potassium Channel Blockers; 0/SLC9A1 protein, human; 0/Sodium Channel Blockers; 0/Sodium-Hydrogen Antiporter; 0/TRPC Cation Channels; 0/TRPC6 protein, human; 147173-20-4/Kv1.1 Potassium Channel; 67256-21-7/Hepatocyte Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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