Document Detail

K-ras mutations, rectal crypt cells proliferation, and meat consumption in patients with left-sided colorectal carcinoma.
MedLine Citation:
PMID:  10777009     Owner:  NLM     Status:  MEDLINE    
It has been suggested that N-nitroso compounds derived from meat may increase the risk of K-ras mutations in the human colon. We sought evidence of associations between red meat consumption, frequency and type of K-ras mutations in resected tumours, and the rate of crypt cell proliferation (CCP) in the normal mucosa of patients with left-sided colorectal carcinoma. Meat consumption was assessed by food frequency questionnaire, and CCP was determined in rectal biopsies obtained prior to surgery. K-ras mutations in the resected tumours were determined using a PCR-based oligonucleotide hybridization assay. Fifteen K-ras mutations were detected in tumours from 43 patients; 13/15 in codon 12, 3/15 in codon 13, and 1/15 in both codons 12 and 13. All mutations were G-->A or G-->T transitions. There was no statistically significant difference between intakes of red meat in patients with a K-ras mutation (92.4 +/- 9.7 g/day) and those without (82.3 +/- 7.7 g/day). Rectal CCP was significantly higher in patients than in healthy controls, but there was no correlation with meat consumption or K-ras mutation. These data do not support the hypothesis that meat consumption is a risk factor for acquisition of K-ras mutations during colorectal carcinogenesis.
H O'Brien; J A Matthew; J M Gee; M Watson; M Rhodes; C T Speakman; W S Stebbings; H J Kennedy; I T Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)     Volume:  9     ISSN:  0959-8278     ISO Abbreviation:  Eur. J. Cancer Prev.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-06-30     Completed Date:  2000-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9300837     Medline TA:  Eur J Cancer Prev     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  41-7     Citation Subset:  IM    
Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, UK.
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MeSH Terms
Cell Division
Cell Transformation, Neoplastic
Colorectal Neoplasms / etiology,  genetics*,  pathology
DNA Mutational Analysis
Genes, ras / genetics*
Middle Aged
Mucous Membrane / cytology
Polymerase Chain Reaction
Rectum / cytology*
Risk Assessment

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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