Document Detail


K-ras gene mutation related to histological atypias in human colorectal adenomas.
MedLine Citation:
PMID:  7578594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the relationship of oncogene analysis to morphology, we analyzed K-ras gene mutations by dot-blot hybridization with and without consideration of histological atypias in individual colorectal adenomas. Each of 54 colon polyps were divided into two parts after fixation. One part was used as a mass to assess point mutations; the remaining portion of each polyp was paraffin-embedded, stained with hematoxylin and eosin, and examined for point mutations related to histological atypias. In the first part of our study, K-ras gene mutations at codon 12 were detected in 13 cases (24%). In the second part of our study, 12 cases had distinctly different histological atypias. From each of these 12 cases, two areas, one with higher or one with lower grade atypia in the same polyp were excised to analyze for K-ras gene mutation. Two of these 12 cases (17%) had the mutation in different areas of the same tumor. These two cases contained the mutation only in the areas with higher grade atypia, and only one case added information regarding ras mutation upon microdissection when compared to the entire biopsy. These results suggest that oligonucleotide hybridization can identify the majority of cases containing ras mutations despite regional morphologic variation. Individual cases, however, may contain clonal subpopulations within adenomas with different ras sequences from other regions within the same adenoma.
Authors:
T Ajiki; T Fujimori; H Ikehara; Y Saitoh; S Maeda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  70     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-11-30     Completed Date:  1995-11-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  90-4     Citation Subset:  IM    
Affiliation:
Second Department of Pathology, Kobe University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenoma / genetics*,  pathology
Adult
Aged
Aged, 80 and over
Base Sequence
Codon
Colon / pathology
Colorectal Neoplasms / genetics*,  pathology
DNA Primers
Disease Progression
Female
Genes, ras*
Humans
Male
Middle Aged
Molecular Sequence Data
Point Mutation*
Polyps / pathology
Chemical
Reg. No./Substance:
0/Codon; 0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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