| K(+)-p-nitrophenylphosphatase activity in rat brain and liver. | |
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MedLine Citation:
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PMID: 18198990 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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K(+)-p-nitrophenylphosphatase (K(+)pNPPase) is the enzyme, which is considered to be involved in K(+)-dependent hydrolysis of the phosphoenzyme in the reaction cycle of Na(+), K(+)ATPase. The aim of our present study was to characterize some features of K(+)pNPPase in homogenates of the rat brain and liver. We determined p-nitrophenylphosphatase (pNPPase) activity in the presence of various ion combinations (Mg(2+)+ K(+), Mg(2+), K(+)). We found a higher total pNPPase activity in the brain (0.8+/-0.079 nkat/mg protein) than in the liver (0.08+/-0.01 nkat/mg protein). Contrary to the liver, the main part of the total brain activity was K(+)-dependent. The activity of K(+)pNPPase was significantly higher in cerebral cortex homogenates (0.86+/-0.073 nkat/mg protein) in comparison to those of the whole brain (0.57+/-0.075 nkat/mg protein). The specific K(+)pNPPase activity was two times higher in the isolated pellet fraction (0.911+/-0.07 nkat/mg protein), rich in synaptosomes, compared to the whole brain homogenate (0.57+/-0.075 nkat/mg protein). Our results demonstrate the high activity of K(+)pNPPase in the brain tissue and its distribution mainly into the pellet fraction, what might indicate a possible role of K(+)pNPPase in specific structures of the brain, e.g. in synaptosomes. |
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Authors:
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M Durfinová; M Brechtlová; B Líska; Z Barosková |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-17 |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: 58 ISSN: 0862-8408 ISO Abbreviation: Physiol Res Publication Date: 2009 |
Date Detail:
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Created Date: 2009-03-31 Completed Date: 2009-06-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: Czech Republic |
Other Details:
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Languages: eng Pagination: 121-6 Citation Subset: IM |
Affiliation:
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Department of Medical Chemistry, Comenius University, Faculty of Medicine, Bratislava, Slovak Republic. durfinova1@post.sk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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4-Nitrophenylphosphatase
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metabolism* Animals Brain / enzymology* Calcium / metabolism Cerebral Cortex / metabolism Liver / enzymology* Magnesium / metabolism Male Potassium / metabolism Rats Rats, Wistar Synaptosomes / enzymology |
| Chemical | |
Reg. No./Substance:
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7439-95-4/Magnesium; 7440-09-7/Potassium; 7440-70-2/Calcium; EC 3.1.3.41/4-Nitrophenylphosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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